CD8 Alpha antibody | 53-6.7

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Rat anti Mouse CD8 Alpha:FITC

Rat anti Mouse CD8 Alpha

Rat anti Mouse CD8 Alpha:RPE

Product Type
Monoclonal Antibody
Clone
53-6.7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2694F F 0.1 mg
MCA2694T C F IF IP 25 µg
MCA2694 C F IF IP 0.25 mg
MCA2694PE F 100 Tests/1ml
Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2. CD8α is the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain.

CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.

Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has also been used for depletion of CD8a+ cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Mouse thymus or spleen.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01731 Related reagents
Entrez Gene
Cd8a Related reagents
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
Regulatory
For research purposes only

Applications of CD8 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Flow Cytometry 1/20
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Immunohistology
MCA2694 has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Western Blotting

Product Specific References

References for CD8 Alpha antibody

  1. Takahashi, K. et al. (1992) CD4 and CD8 regulate interleukin 2 responses of T cells.
    Proc Natl Acad Sci U S A. 89 (12): 5557-61.
  2. Shore, D.A. et al. (2008) The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.
    J Mol Biol. 384 (5): 1190-202.
  3. McDole, J.R. et al. (2010) Rapid formation of extended processes and engagement of Theiler's virus-infected neurons by CNS-infiltrating CD8 T cells.
    Am J Pathol. 177 (4): 1823-33.
  4. Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
    J Clin Invest. 125 (11): 4281-94.
  5. Johnson, H.L. et al. (2014) Perforin competent CD8 T cells are sufficient to cause immune-mediated blood-brain barrier disruption.
    PLoS One. 9 (10): e111401.
  6. Schuster, C. et al. (2013) A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.
    PLoS One. 8 (8): e71746.

Further Reading

  1. Jabbari, A. & Harty, J.T. (2006) The generation and modulation of antigen-specific memory CD8 T cell responses.
    J Leukoc Biol. 80 (1): 16-23.