CD38 antibody | 90

Rat anti Mouse CD38

Product Type
Monoclonal Antibody
Clone
90
Isotype
IgG2a
Specificity
CD38

Product Code Applications Pack Size List Price Your Price Qty
MCA1511R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Rat anti Mouse CD38 antibody, clone 90 recognizes the murine CD38 cell surface antigen, a ~42-46kDa glycoprotein expressed by B lymphocytes, a proportion of peripheral T lymphocytes and by peritoneal macrophages.

In the mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse pre-B cells derived from IL-7 dependent bone marrow cultures.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Lymph node cells from an immunized rat were fused with cells of the mouse P3X63Ag.8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2 1/20
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD38 antibody

  1. Lund, F. et al. (1995) Murine CD38: an immunoregulatory ectoenzyme.
    Immunol Today. 16 (10): 469-73.
  2. Oliver, A.M. et al. (1997) Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.
    J Immunol. 158 (3): 1108-15.

RRID
AB_2073336
UniProt
P56528
Entrez Gene
Cd38
GO Terms
GO:0003953 NAD+ nucleosidase activity
GO:0016021 integral to membrane
GO:0005488 binding
GO:0009986 cell surface
GO:0016849 phosphorus-oxygen lyase activity
GO:0030890 positive regulation of B cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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