CD34 antibody | MEC14.7

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Rat anti Mouse CD34:FITC

Rat anti Mouse CD34:Low Endotoxin

Rat anti Mouse CD34

Rat anti Mouse CD34:RPE

Product Type
Monoclonal Antibody
Clone
MEC14.7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1825F F 0.1 mg
MCA1825FA F 50 µg
MCA1825FB F 0.5 mg
MCA1825EL F IP P 0.5 mg
MCA1825GA F IP P 0.1 mg
MCA1825T F IP P 25 µg
MCA1825 F IP P 0.25 mg
MCA1825PE F 100 Tests
MCA1825PET F 25 Tests
Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1.0 ml distilled water
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
T-end.1, a pMT transformed endothelial cell line.
Approx. Protein Concentrations
Pack Size: 0.1 mg, 50 µg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml

Storage Information

Storage
Pack Size: 0.1 mg, 50 µg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q64314 Related reagents
Entrez Gene
Cd34 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0009897 external side of plasma membrane
GO:0043199 sulfate binding
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of CD34 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 Neat Pack Size: 0.1 mg
1/10 Pack Size: 0.5 mg
Flow Cytometry
Immunohistology - Paraffin 1/20 1/200
Immunoprecipitation
Flow Cytometry 1/25 Pack Size: 0.1 mg
1/10 Pack Size: 25 µg, 0.25 mg
1/50 Pack Size: 0.1 mg
1/20 Pack Size: 25 µg, 0.25 mg
Immunohistology - Paraffin 1/20 1/200
Immunoprecipitation 2ug/ml 10ug/ml
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL 0.5 mg F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

Source Reference

  1. Garlanda, C. et al. (1997) Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
    Eur J Cell Biol. 73 (4): 368-77.

References for CD34 antibody

  1. Winding, B. et al. (2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.
    Clin Cancer Res. 8 (6): 1932-9.
  2. Nguyen, L. et al. (2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.
    BMC Cancer. 12: 522.
  3. Morison, N.B. et al. (2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.
    Reproduction. 133: 309-21.
  4. Chen, L. et al. (2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.
    Am J Pathol. 177: 2671-80.
  5. Ager, E.I. et al. (2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.
    Cancer Cell Int. 10: 19
  6. Chabot, S. et al. (2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.
    J Exp Med. 208: 973-86.
  7. Chen, J. et al. (2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.
    Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71.
  8. Chen, J. et al. (2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.
    PLoS One. 7 (11): e50105.
  9. Ouji Y & Yoshikawa M (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.
    Methods Mol Biol. Apr 1. [Epub ahead of print]
  10. Nguyen, L. et al. (2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.
    BMC Cancer. 16 (1): 533.
  11. Vávrová, J. et al. (2012) Irradiated stem cells and ageing of the haematopoietic system.
    Radiat Environ Biophys. 51 (2): 205-13.
  12. DaCostaP, L.N. et al. (2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.
    BMC Cancer. 18 (1): 382.