CD34 antibody | MEC14.7












Rat anti Mouse CD34:FITC
Rat anti Mouse CD34:Low Endotoxin
Rat anti Mouse CD34
Rat anti Mouse CD34:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MEC14.7
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1825F | F | 0.1 mg |
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MCA1825FA | F | 50 µg |
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MCA1825FB | F | 0.5 mg |
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MCA1825EL | F IP P | 0.5 mg |
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MCA1825 | F IP P | 0.25 mg |
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MCA1825T | F IP P | 25 µg |
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MCA1825GA | F IP P | 0.1 mg |
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MCA1825PE | F | 100 Tests |
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Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- T-end.1, a pMT transformed endothelial cell line.
- Approx. Protein Concentrations
- Pack Size: 0.1 mg, 50 µgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
Storage Information
- Storage
- Pack Size: 0.1 mg, 50 µgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q64314
- Entrez Gene
- Cd34
- GO Terms
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005737 cytoplasm
- GO:0009897 external side of plasma membrane
- GO:0043199 sulfate binding
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of CD34 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | Neat Pack Size: 0.1 mg 1/10 Pack Size: 0.5 mg |
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Flow Cytometry | |||
Immunohistology - Paraffin | 1/20 | 1/200 | |
Immunoprecipitation | |||
Flow Cytometry | 1/10 Pack Size: 0.25 mg, 25 µg 1/25 Pack Size: 0.1 mg |
1/20 Pack Size: 0.25 mg, 25 µg 1/50 Pack Size: 0.1 mg |
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Immunohistology - Paraffin | 1/20 | 1/200 | |
Immunoprecipitation | 2ug/ml | 10ug/ml | |
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests |
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Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | F | 0.5 mg |
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Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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Product Specific References
Source Reference
-
Garlanda, C. et al. (1997) Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
Eur J Cell Biol. 73 (4): 368-77.
References for CD34 antibody
-
Winding, B. et al. (2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.
Clin Cancer Res. 8 (6): 1932-9. -
Nguyen, L. et al. (2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.
BMC Cancer. 12: 522. -
Morison, N.B. et al. (2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.
Reproduction. 133: 309-21. -
Chen, L. et al. (2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.
Am J Pathol. 177: 2671-80. -
Ager, E.I. et al. (2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.
Cancer Cell Int. 10: 19 -
Chabot, S. et al. (2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.
J Exp Med. 208: 973-86. -
Chen, J. et al. (2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.
Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71. -
Chen, J. et al. (2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.
PLoS One. 7 (11): e50105. -
Ouji, Y. & Yoshikawa, M. (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.
Methods Mol Biol. 1516: 279-88. -
Nguyen, L. et al. (2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.
BMC Cancer. 16 (1): 533. -
Vávrová, J. et al. (2012) Irradiated stem cells and ageing of the haematopoietic system.
Radiat Environ Biophys. 51 (2): 205-13. -
DaCosta, P.L.N. et al. (2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.
BMC Cancer. 18 (1): 382. -
Danielyan, L. et al.. (2020) Cell motility and migration as determinants of stem cell efficacy
EBioMedicine. 60:102989. -
Rackham, C.L. et al. (2013) Maintenance of islet morphology is beneficial for transplantation outcome in diabetic mice.
PLoS One. 8 (2): e57844. -
Fruchon, S. et al. (2012) Involvement of the Syk-mTOR pathway in follicular lymphoma cell invasion and angiogenesis.
Leukemia. 26 (4): 795-805.
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