CD34 antibody | MEC14.7

Product Code Applications Pack Size List Price Your Price Qty
MCA1825
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SDS Safety Datasheet SDS
F IP P 0.25 mg loader
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loader
MCA1825T
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SDS Safety Datasheet SDS
F IP P 25 µg loader
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loader
MCA1825GA
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F IP P 0.1 mg loader
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Search for Batch Specific Datasheets

Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
T-end.1, a pMT transformed endothelial cell line.
Approx. Protein Concentrations
Pack Size: 0.25 mg, 25 µg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 Pack Size: 0.25 mg, 25 µg
1/25 Pack Size: 0.1 mg
1/20 Pack Size: 0.25 mg, 25 µg
1/50 Pack Size: 0.1 mg
Immunohistology - Paraffin 1/20 1/200
Immunoprecipitation 2ug/ml 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Garlanda, C. et al. (1997) Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors.
    Eur J Cell Biol. 73 (4): 368-77.

References for CD34 antibody

  1. Winding, B. et al. (2002) Synthetic matrix metalloproteinase inhibitors inhibit growth of established breast cancer osteolytic lesions and prolong survival in mice.
    Clin Cancer Res. 8 (6): 1932-9.
  2. Nguyen, L. et al. (2012) Spatial morphological and molecular differences within solid tumors may contribute to the failure of vascular disruptive agent treatments.
    BMC Cancer. 12: 522.
  3. Morison, N.B. et al. (2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.
    Reproduction. 133: 309-21.
  4. Chen, L. et al. (2010) Roles of tetrahydrobiopterin in promoting tumor angiogenesis.
    Am J Pathol. 177: 2671-80.
  5. Ager, E.I. et al. (2010) Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases.
    Cancer Cell Int. 10: 19
  6. Chabot, S. et al. (2011) A novel antiangiogenic and vascular normalization therapy targeted against human CD160 receptor.
    J Exp Med. 208: 973-86.
  7. Chen, J. et al. (2011) Circulating endothelial progenitor cells and cellular membrane microparticles in db/db diabetic mouse: possible implications in cerebral ischemic damage.
    Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E62-71.
  8. Chen, J. et al. (2012) Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.
    PLoS One. 7 (11): e50105.
  9. View The Latest Product References
  10. Ouji, Y. & Yoshikawa, M. (2016) Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro.
    Methods Mol Biol. 1516: 279-88.
  11. Nguyen, L. et al. (2016) Vascular disruptive agent OXi4503 and anti-angiogenic agent Sunitinib combination treatment prolong survival of mice with CRC liver metastasis.
    BMC Cancer. 16 (1): 533.
  12. Vávrová, J. et al. (2012) Irradiated stem cells and ageing of the haematopoietic system.
    Radiat Environ Biophys. 51 (2): 205-13.
  13. DaCosta, P.L.N. et al. (2018) The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model.
    BMC Cancer. 18 (1): 382.
  14. Danielyan, L. et al.. (2020) Cell motility and migration as determinants of stem cell efficacy
    EBioMedicine. 60:102989.
  15. Rackham, C.L. et al. (2013) Maintenance of islet morphology is beneficial for transplantation outcome in diabetic mice.
    PLoS One. 8 (2): e57844.
  16. Fruchon, S. et al. (2012) Involvement of the Syk-mTOR pathway in follicular lymphoma cell invasion and angiogenesis.
    Leukemia. 26 (4): 795-805.
  17. Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
    J Neuroinflammation. 17 (1): 85.

Immunohistology - Paraffin

RRID
AB_322537
UniProt
Q64314
Entrez Gene
Cd34
GO Terms
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0009897 external side of plasma membrane
GO:0043199 sulfate binding
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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