CD283 antibody | 11F8

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Rat anti Mouse CD283:FITC

Rat anti Mouse CD283

Product Type
Monoclonal Antibody
Clone
11F8
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5891F F IF 0.1 mg
MCA5891GA F IF 0.1 mg
Rat anti Mouse CD283 antibody, clone 11F8 recognizes mouse TLR3, otherwise known as CD283 or Toll-like receptor 3. CD283 is a 905 amino acid ~105 kDa single pass type 1 transmembrane glycoprotein associated with endosomal membranes and member of the Toll-like receptor (TLR) family. CD283 functions as a receptor for double-stranded RNA (UniProt: Q99MB1).
TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. TLRs also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide.
Carrier Free
Yes
Immunogen
Purified murine TLR3 extracellular domain protein.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q99MB1 Related reagents
Entrez Gene
Tlr3 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003725 double-stranded RNA binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005789 endoplasmic reticulum membrane
GO:0006954 inflammatory response
GO:0010008 endosome membrane
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032728 positive regulation of interferon-beta production
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0034346 positive regulation of type III interferon production
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043330 response to exogenous dsRNA
GO:0045078 positive regulation of interferon-gamma biosynthetic process
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045087 innate immune response
GO:0045356 positive regulation of interferon-alpha biosynthetic process
GO:0045359 positive regulation of interferon-beta biosynthetic process
GO:0046330 positive regulation of JNK cascade
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of CD283 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Immunofluorescence
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Leucoperm™ BUF09 50 Tests F
Leucoperm™ BUF09 50 Tests F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD283 antibody

  1. McCartney, S. et al. (2009) Distinct and complementary functions of MDA5 and TLR3 in poly(I:C)-mediated activation of mouse NK cells.
    J Exp Med. 206: 2967-76.
  2. Jelinek, I. et al. (2011) TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection.
    J Immunol. 186: 2422-9.
  3. Azuma, M. et al. (2012) Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8α(+) dendritic cells.
    Oncoimmunology. 1: 581-592.
  4. McCartney, S.A. et al. (2011) RNA sensor–induced type I IFN prevents diabetes caused by a β cell–tropic virus in mice
    J Clin Invest. 121: 1497-507.
  5. Zeng, R. et al. (2016) Generation and transcriptional programming of intestinal dendritic cells: essential role of retinoic acid.
    Mucosal Immunol. 9 (1): 183-93.