CD283 antibody | 11F8
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F ResetRat anti Mouse CD283
- Product Type
- Monoclonal Antibody
- Clone
- 11F8
- Isotype
- IgG2a
- Specificity
- CD283
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD283 antibody, clone 11F8 recognizes mouse TLR3, otherwise known as CD283 or Toll-like receptor 3. CD283 is a 905 amino acid ~105 kDa single pass type 1 transmembrane glycoprotein associated with endosomal membranes and member of the Toll-like receptor (TLR) family. CD283 functions as a receptor for double-stranded RNA (UniProt: Q99MB1). TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. TLRs also initiate cell signaling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide.
- Carrier Free
- Yes
- Immunogen
- Purified murine TLR3 extracellular domain protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD283 antibody
-
McCartney, S. et al. (2009) Distinct and complementary functions of MDA5 and TLR3 in poly(I:C)-mediated activation of mouse NK cells.
J Exp Med. 206: 2967-76. -
Jelinek, I. et al. (2011) TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection.
J Immunol. 186: 2422-9. -
Azuma, M. et al. (2012) Cross-priming for antitumor CTL induced by soluble Ag + polyI:C depends on the TICAM-1 pathway in mouse CD11c(+)/CD8α(+) dendritic cells.
Oncoimmunology. 1: 581-592. -
McCartney, S.A. et al. (2011) RNA sensor–induced type I IFN prevents diabetes caused by a β cell–tropic virus in mice
J Clin Invest. 121: 1497-507. -
Zeng, R. et al. (2016) Generation and transcriptional programming of intestinal dendritic cells: essential role of retinoic acid.
Mucosal Immunol. 9 (1): 183-93. -
Njoroge, J.M. et al. (2018) A flow cytometric analysis of macrophage- nanoparticle interactions in vitro: induction of altered Toll-like receptor expression.
Int J Nanomedicine. 13: 8365-78.
- Synonyms
- TLR3
- UniProt
- Q99MB1
- Entrez Gene
- Tlr3
- GO Terms
- GO:0005515 protein binding
- GO:0003725 double-stranded RNA binding
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005789 endoplasmic reticulum membrane
- GO:0006954 inflammatory response
- GO:0010008 endosome membrane
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032728 positive regulation of interferon-beta production
- View More GO Terms
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0034346 positive regulation of type III interferon production
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043330 response to exogenous dsRNA
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0045080 positive regulation of chemokine biosynthetic process
- GO:0045087 innate immune response
- GO:0045356 positive regulation of interferon-alpha biosynthetic process
- GO:0045359 positive regulation of interferon-beta biosynthetic process
- GO:0046330 positive regulation of JNK cascade
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051607 defense response to virus
MCA5891GA
153429
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