CD200 antibody | OX-90

Rat anti Mouse CD200

Product Type
Monoclonal Antibody
Clone
OX-90
Isotype
IgG2a
Specificity
CD200

Product Code Applications Pack Size List Price Your Price Qty
MCA1958GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F 0.1 mg loader
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loader
MCA1958
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F 0.25 mg loader
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loader
MCA1958T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2.

CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.

The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Mouse CD200-rat CD4 fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Rat IgG2a Negative Control

References for CD200 antibody

  1. Hoek, R.M. et al. (2000) Down-regulation of the macrophage lineage through interaction with OX2 (CD200).
    Science. 290 (5497): 1768-71.
  2. Nathan, C. & Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200?
    Nat Immunol. 2 (1): 17-9.
  3. Broderick, C. et al. (2002) Constitutive retinal CD200 expression regulates resident microglia and activation state of inflammatory cells during experimental autoimmune uveoretinitis.
    Am J Pathol. 161 (5): 1669-77.
  4. Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
    Int Immunopharmacol. 11 (7): 794-801.
  5. Burger, P.E. et al. (2009) High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
    Stem Cells. 27: 2220-8.
  6. Garza, L.A. et al. (2011) Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.
    J Clin Invest. 121: 613-22.
  7. Ko, Y.C. et al (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    J Anat. 214: 183-95.
  8. Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
    J Neuropathol Exp Neurol. 68: 159-67.
  9. View The Latest Product References
  10. Krejsek, J. et al (2010) Expression of CD200/CD200R regulatory molecules on granulocytes and monocytes is modulated by cardiac surgical operation.
    Perfusion. 25: 389-97.
  11. Rijkers, E.S. (2007) Ligation of CD200R by CD200 is not required for normal murine myelopoiesis.
    Eur J Haematol. 79: 410-6.
  12. Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
    Mol Immunol. 45: 1126-35.
  13. Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
    J Immunol. Jul 6. pii: 1600052. [Epub ahead of print]
  14. Montiel, M. et al. (2016) Melatonin decreases brain apoptosis, oxidative stress, and CD200 expression and increased survival rate in mice infected by Venezuelan equine encephalitis virus.
    Antivir Chem Chemother. Aug 8. pii: 2040206616660851. [Epub ahead of print]
  15. Liu, C. et al. (2018) The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation.
    J Inflamm (Lond). 15: 28.
  16. Tonecka, K. et al. (2021) The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
    Int J Mol Sci. 22 (10)May 19 [Epub ahead of print].
  17. Pannunzio, B. et al. (2022) CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
    Cells. 11 (11): 1786.

Flow Cytometry

Immunohistology - Frozen

Synonyms
OX2
RRID
AB_323317
UniProt
O54901
Entrez Gene
Cd200
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
View more products with CD200 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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