CD200 antibody | OX-90
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to block CD200 interactions by incubation in vitro with murine BV2 cells.
Image caption:
N44Q mutant involved in CD200-CD200R1 interaction and LPS-induced classical microglial activation. We established neuronal-microglia co-cultures to explore the effect of N44Q mutant. a, b LPS significantly increased iNOS mRNA and CD86 mRNA in BV2 cells. The addition of neurons attenuated LPS-induced classical microglial activation. Notably, the present of anti-CD200 antibody blocked the modulating effect of neurons. BV2 cells transfected with N44Q mutant exerted similar effects like anti-CD200 antibody (*p < 0.01; +p < 0.01; #p < 0.01, WT + LPS + neurons vs WT + LPS; **p < 0.01; N44Q + LPS + neurons vs N44Q + LPS; ++p < 0.01, N44Q + LPS + neurons + anti-CD200 antibody vs N44Q + LPS + neurons; ANOVA; n = 5). c, d IL-1β mRNA and TNF-α mRNA were examined. The LPS-induced effects were reversed in the presence of neurons. Importantly, the addition of anti-CD200 antibody abrogated the modulating effect of neurons, consistent with the cells transfected with N44Q mutant (*p < 0.01; +p < 0.01; #p < 0.05, WT + LPS + neurons vs WT + LPS; **p < 0.01; N44Q + LPS + neurons vs N44Q + LPS; ++p < 0.01, N44Q + LPS + neurons + anti-CD200 antibody vs N44Q + LPS + neurons; ANOVA; n = 5). Similar results were obtained by ELISA (*p < 0.01; +p < 0.01; #p < 0.01, WT + LPS + neurons vs WT + LPS; **p < 0.01; N44Q + LPS + neurons vs N44Q + LPS; ++p < 0.01, N44Q + LPS + neurons + anti-CD200 antibody vs N44Q + LPS + neurons; ANOVA; n = 5). Error bars indicate ± SEM
From: Liu C, Shen Y, Tang Y, Gu Y.
The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation.
J Inflamm (Lond). 2018;15:28
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to demonstrate CD200 expression in mouse colonic tissue by immunofluorescence.
Image caption:
CD200 expression within the intestine. (A) Immunofluorescence staining for CD200 (green) in colon tissue from untreated mice. Cell nuclei were visualized by DAPI (blue). Intense CD200 immunostaining was observed in the subepithelial layer of the colonic villi (magnification 40× or 20×), scale bar = 100 μm;
From: Tonecka K, Braniewska A, Pilch Z, Sas Z, Skorzynski M, Manuali E, Rygiel TP.
The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
Int J Mol Sci. 2021 May 19;22(10):5358.
doi: 10.3390/ijms22105358.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to label cells in mouse sciatic nerve following injury by immunofluorescence of formalin fixed cryosections.
Image caption:
Expression of CD200 in naive and lesioned nerve. Single one micrometer confocal planes of CD200 immunohistochemistry in longitudinal sections of the sciatic nerve show CD200 protein expression in the Ranvier Nodes (arrow) and blood vessels (arrowhead) ((A–F), S100-B is a Schwann cells marker, and the tomato lectin is an endothelial cell marker). Confocal images of teased fibers of adult sciatic nerve show CD200 expression at the Ranvier Nodes (arrow) and at the Schmidt–Lanterman incisures (arrowheads) (G–I). CD200 mRNA expression in naive nerve and after sciatic nerve crush injury at different days post-injury (dpi) (J) (One-way ANOVA followed by Newman–Keuls post-hoc test * p ≤0.05; ** p ≤ 0.01; n = 3–4 animals per time point).
From: Pannunzio, B.; Amo-Aparicio, J.; Julián, C.; López-Vales, R.; Peluffo, H.; Lago, N.
CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury.
Cells 2022, 11, 1786.
doi: 10.3390/cells11111786.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to label cells in mouse sciatic nerve following injury by immunofluorescence of formalin fixed cryosections.
Image caption:
Expression of CD200 in naive and lesioned nerve. Single one micrometer confocal planes of CD200 immunohistochemistry in longitudinal sections of the sciatic nerve in naive conditions and after crush sciatic nerve show a marked reduction in CD200 protein expression at one day post-injury and an increase in the expression from seven days post-injury (K–N). Scale bars: 30 μm.
From: Pannunzio, B.; Amo-Aparicio, J.; Julián, C.; López-Vales, R.; Peluffo, H.; Lago, N.
CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury.
Cells 2022, 11, 1786.
doi: 10.3390/cells11111786.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD200
- Product Type
- Monoclonal Antibody
- Clone
- OX-90
- Isotype
- IgG2a
- Specificity
- CD200
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2. CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons. The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Mouse CD200-rat CD4 fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
1/50 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Mouse spleen
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
References for CD200 antibody
-
Hoek, R.M. et al. (2000) Down-regulation of the macrophage lineage through interaction with OX2 (CD200).
Science. 290 (5497): 1768-71. -
Nathan, C. & Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200?
Nat Immunol. 2 (1): 17-9. -
Rijkers, E.S. (2007) Ligation of CD200R by CD200 is not required for normal murine myelopoiesis.
Eur J Haematol. 79: 410-6. -
Rijkers, E.S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes.
Mol Immunol. 45: 1126-35. -
Burger, P.E. et al. (2009) High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
Stem Cells. 27: 2220-8. -
Ko, Y.C. et al (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95. -
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67. -
Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
Int Immunopharmacol. 11 (7): 794-801.
View The Latest Product References
-
Garza, L.A. et al. (2011) Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.
J Clin Invest. 121: 613-22. -
Montiel, M. et al. (2015) Melatonin decreases brain apoptosis, oxidative stress, and CD200 expression and increased survival rate in mice infected by Venezuelan equine encephalitis virus.
Antivir Chem Chemother. 24 (3-4): 99-108. -
Liu, J.Q. et al. (2016) A Critical Role for CD200R Signaling in Limiting the Growth and Metastasis of CD200+ Melanoma.
J Immunol. 197 (4): 1489-97. -
Liu, C. et al. (2018) The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation.
J Inflamm (Lond). 15: 28. -
Lago, N. et al. (2018) CD200 modulates spinal cord injury neuroinflammation and outcome through CD200R1.
Brain Behav Immun. 73: 416-26. -
Tonecka, K. et al. (2021) The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
Int J Mol Sci. 22 (10): 5358. -
Pannunzio, B. et al. (2022) CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
Cells. 11 (11): 1786.
- Synonyms
- OX2
- RRID
- AB_323317
- UniProt
- O54901
- Entrez Gene
- Cd200
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
View more products with CD200 specificity
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