CD13 antibody | ER-BMDM1
Rat anti Mouse CD13
- Product Type
- Monoclonal Antibody
- Clone
- ER-BMDM1
- Isotype
- IgG2a
- Specificity
- CD13
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD13 antibody, clone ER-BMDM1 recognizes the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity. In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13. Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Balb/c bone marrow derived macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Leenen, P.J. et al. (1992) The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity.
Eur J Immunol. 22 (6): 1567-72.
References for CD13 antibody
-
Leenen, P.J. et al. (1998) Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.
J Immunol. 160 (5): 2166-73. -
Sen, A. et al. (2012) Innate immune response to homologous rotavirus infection in the small intestinal villous epithelium at single-cell resolution.
Proc Natl Acad Sci U S A. 109: 20667-72. -
Onogi, Y. et al. (2017) PDGFRβ Regulates Adipose Tissue Expansion and Glucose Metabolism via Vascular Remodeling in Diet-Induced Obesity.
Diabetes. 66 (4): 1008-21. -
Rudnicki, M. et al. (2022) Quantitative Methods to Assess Adipose Vasculature.
Methods Mol Biol. 2441: 201-21.
Further Reading
-
van der Velden, V.H. et al. (2001) CD13/aminopeptidase N involvement in dendritic cell maturation.
Leukemia. 15 (1): 190-1.
- Synonyms
- Aminopeptidase N
- RRID
- AB_609577
- UniProt
- P97449
- Entrez Gene
- Anpep
- GO Terms
- GO:0001525 angiogenesis
- GO:0004177 aminopeptidase activity
- GO:0008237 metallopeptidase activity
- GO:0016021 integral to membrane
- GO:0008270 zinc ion binding
- GO:0030154 cell differentiation
MCA2395T
167630
1702
MCA2395
155993
1609
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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