CD13 antibody | ER-BMDM1

Rat anti Mouse CD13

Product Type
Monoclonal Antibody
Clone
ER-BMDM1
Isotype
IgG2a
Specificity
CD13

Product Code Applications Pack Size List Price Your Price Qty
MCA2395
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
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loader
MCA2395T
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SDS Safety Datasheet SDS
C F 25 µg loader
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Rat anti Mouse CD13 antibody, clone ER-BMDM1 recognizes the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.

In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.

Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balb/c bone marrow derived macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for CD13 antibody

  1. Leenen, P.J. et al. (1992) The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity.
    Eur J Immunol. 22 (6): 1567-72.
  2. Leenen, P.J. et al. (1998) Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.
    J Immunol. 160 (5): 2166-73.
  3. Sen, A. et al. (2012) Innate immune response to homologous rotavirus infection in the small intestinal villous epithelium at single-cell resolution.
    Proc Natl Acad Sci U S A. 109: 20667-72.
  4. Onogi, Y. et al. (2017) PDGFRβ Regulates Adipose Tissue Expansion and Glucose Metabolism via Vascular Remodeling in Diet-Induced Obesity.
    Diabetes. 66 (4): 1008-21.
  5. Rudnicki, M. et al. (2022) Quantitative Methods to Assess Adipose Vasculature.
    Methods Mol Biol. 2441: 201-21.

Further Reading

  1. van der Velden, V.H. et al. (2001) CD13/aminopeptidase N involvement in dendritic cell maturation.
    Leukemia. 15 (1): 190-1.

Synonyms
Aminopeptidase N
RRID
AB_609577
UniProt
P97449
Entrez Gene
Anpep
GO Terms
GO:0001525 angiogenesis
GO:0004177 aminopeptidase activity
GO:0008237 metallopeptidase activity
GO:0016021 integral to membrane
GO:0008270 zinc ion binding
GO:0030154 cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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