Infliximab antibody | AbD19376

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Human anti Infliximab

Anti-infliximab antibody is a non-neutralizing anti-idiotypic monovalent Fab antibody; it is ideal for bioanalytical assays for infliximab and biosimilars. It does not inhibit the binding of infliximab to TNF alpha and so detects total drug. It is recommended as PK bridging ELISA capture antibody.

Product Type
Monoclonal Antibody
Clone
AbD19376
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA214 E 0.1 mg
HCA214G E 1 mg
Human Anti-Infliximab Antibody, clone AbD19376 is an anti-idiotypic antibody that specifically recognizes the infliximab monoclonal antibody and does not inhibit the binding of infliximab to its target, tumor necrosis factor alpha (TNFα). It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples.

Clone AbD19376 recognizes both the free infliximab monoclonal antibody and infliximab when bound to TNFα (the drug/target complex); it is not paratope-specific and it is non-inhibitory. This antibody can be used as a capture antibody in a pharmacokinetic (PK) bridging ELISA to detect total drug (free, partially bound and fully bound) with HCA216P as the detection antibody.

Infliximab (branded as Remicade) is a chimeric monoclonal antibody drug (IgG1/kappa) that has been approved for treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, and ulcerative colitis. It acts by blocking the binding of TNFα to its receptors. Infliximab also induces apoptosis of TNFα expressing T-lymphocytes.

View a summary of all Anti-Infliximab Antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Infliximab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.7 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Remicade is a registered trademark of Janssen Biotech Inc. Inflectra is a registered trademark of Hospira, a Pfizer Company. RENFLEXIS is a trademark of Merck Sharp & Dohme Corp.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Infliximab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or as a capture antibody in an Infliximab bridging ELISA together HRP-conjugated HCA213 or HCA216 as the detection reagent.
Protocol: PK bridging ELISA to measure total drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P 0.1 mg E WB
Rat anti DYKDDDDK Tag:HRP MCA4764P 0.1 mg E P WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Histidine Tag:Biotin AHP1656B 0.1 mg E WB
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Infliximab HCA213 0.1 mg E
Human anti Infliximab HCA215 0.1 mg E
Human anti Infliximab HCA216 0.1 mg E
Mouse anti Histidine Tag:Alk.Phos. MCA1396A 0.1 mg E WB
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P 0.2 mg C* E
Recombinant Human TNF Alpha PHP051 50 µg E FN WB