Infliximab antibody | AbD19376

Human anti Infliximab

Product Type
Monoclonal Antibody
Clone
AbD19376
Isotype
HuCAL Fab monovalent
Specificity
Infliximab

Product Code Applications Pack Size List Price Your Price Qty
HCA214G
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SDS Safety Datasheet SDS
E 1 mg loader
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HCA214
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E 0.1 mg loader
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Human Anti-Infliximab Antibody, clone AbD19376 is an anti-idiotypic antibody that specifically recognizes the infliximab monoclonal antibody and does not inhibit the binding of infliximab to its target, tumor necrosis factor alpha (TNFα). It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples.

Clone AbD19376 recognizes both the free infliximab monoclonal antibody and infliximab when bound to TNFα (the drug/target complex); it is not paratope-specific and it is non-inhibitory. This antibody can be used as a capture antibody in a pharmacokinetic (PK) bridging ELISA to detect total drug (free, partially bound and fully bound) with HCA216P as the detection antibody.

Infliximab (branded as Remicade) is a chimeric monoclonal antibody drug (IgG1/kappa) that has been approved for treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, and ulcerative colitis. It acts by blocking the binding of TNFα to its receptors. Infliximab also induces apoptosis of TNFα expressing T-lymphocytes.

View a summary of all Anti-Infliximab Antibodies.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Infliximab
Affinity
The monovalent intrinsic affinity of this antibody is KD=0.7 nM as measured by real time, label-free molecular interaction analysis on immobilized infliximab.
Approx. Protein Concentrations
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Remicade is a trademark of Janssen Biotech Inc. Inflectra is a trademark of Hospira, a Pfizer Company. RENFLEXIS is a trademark of Merck Sharp & Dohme Corp.
His-tag is a trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in an Infliximab bridging ELISA together HRP-conjugated HCA213 or HCA216 as the detection reagent.
Protocol: PK bridging ELISA to measure total drug.

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Description Rat anti DYKDDDDK Tag:HRP
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Description Rabbit anti DYKDDDDK Tag:HRP

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Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
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Description Goat anti Histidine Tag:Biotin
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
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Description Recombinant Human TNF Alpha
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Infliximab HCA216 E 0.1 mg loader
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Description Human anti Infliximab
Human anti Infliximab HCA215 E 0.1 mg loader
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Description Human anti Infliximab
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg loader
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Description Mouse anti Histidine Tag:Alk.Phos.
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
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Description Mouse anti Histidine Tag:Biotin
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP
Human anti Infliximab HCA213 E 0.1 mg loader
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Description Human anti Infliximab

References for Infliximab antibody

  1. Bar-Yoseph, H et al () Differential serum-intestinal dynamics of infliximab and adalimumab in inflammatory bowel disease patients
    J Chron's Colitis.

Synonyms
Remicade
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"