TNF Alpha antibody | MP9-20A4

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Rat anti Human TNF Alpha:Preservative Free

Rat anti Human TNF Alpha

Rat anti Human TNF Alpha:RPE

Product Type
Monoclonal Antibody
Clone
MP9-20A4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1560XZ C E F* FN 1 mg
MCA1560 C E F* 0.5 mg
MCA1560PE F* 100 Tests
Rat anti Human TNF alpha antibody, clone MP9-20A4 recognizes human tumor Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. This ~17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.

Rat anti Human TNF alpha antibody, clone MP9-20A4 is reported to act as a blocking antibody (Abrams et al. 2001).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01375 Related reagents
Entrez Gene
TNF Related reagents
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0000185 activation of MAPKKK activity
GO:0000187 activation of MAPK activity
GO:0001891 phagocytic cup
GO:0002439 chronic inflammatory response to antigenic stimulus
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0009615 response to virus
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0006919 activation of caspase activity
GO:0006927 transformed cell apoptosis
GO:0050796 regulation of insulin secretion
GO:0042802 identical protein binding
GO:0009651 response to salt stress
GO:0009897 external side of plasma membrane
GO:0010843 promoter binding
GO:0010888 negative regulation of lipid storage
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0030730 sequestering of triglyceride
GO:0031334 positive regulation of protein complex assembly
GO:0031622 positive regulation of fever generation
GO:0032582 negative regulation of gene-specific transcription
GO:0032715 negative regulation of interleukin-6 production
GO:0032722 positive regulation of chemokine production
GO:0032800 receptor biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043193 positive regulation of gene-specific transcription
GO:0043243 positive regulation of protein complex disassembly
GO:0045071 negative regulation of viral genome replication
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045121 membrane raft
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045672 positive regulation of osteoclast differentiation
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050715 positive regulation of cytokine secretion
GO:0050901 leukocyte tethering or rolling
GO:0050995 negative regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0055037 recycling endosome
GO:0060555 induction of necroptosis by extracellular signals
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0061048 negative regulation of branching involved in lung morphogenesis
GO:0070265 necrotic cell death
GO:0070374 positive regulation of ERK1 and ERK2 cascade
Regulatory
For research purposes only

Applications of TNF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml 10ug/ml
Flow Cytometry 1
Functional Assays
Immunohistology - Frozen
Western Blotting
ELISA 5ug/ml 10ug/ml
Flow Cytometry 1
Immunohistology - Frozen
Western Blotting
Flow Cytometry 1 Neat
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. 9&vers=NA">BUF09) for this purpose.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA6004GA 0.1 mg F
Rat IgG1 Negative Control MCA6004GA 0.1 mg F
Rat IgG1 Negative Control:RPE MCA6004PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for TNF Alpha antibody

  1. Butler, D.M. et al. (1997) DBA/1 mice expressing the human TNF-alpha transgene develop a severe, erosive arthritis: characterization of the cytokine cascade and cellular composition.
    J Immunol. 159 (6): 2867-76.
  2. Vujanovic, L. et al. (2010) Virally infected and matured human dendritic cells activate natural killer cells via cooperative activity of plasma membrane-bound TNF and IL-15.
    Blood. 116: 575-83.
  3. Abrams, J.S. (2001) Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies.
    Curr Protoc Immunol. Chapter 6: Unit 6.20.
  4. Evans HG et al. (2009) In vivo activated monocytes from the site of inflammation in humans specifically promote Th17 responses.
    Proc Natl Acad Sci U S A. 106 (15): 6232-7.
  5. Kobawala TP et al. (2016) Significance of TNF-α and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma.
    J Thyroid Res. 2016: 8143695.
  6. Mann, E.R. et al. (2016) Compartment-specific immunity in the human gut: properties and functions of dendritic cells in the colon versus the ileum.
    Gut. 65 (2): 256-70.
  7. Kumagai, M. et al. (2012) Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.
    J Control Release. 160 (3): 542-51.
  8. Poussin C et al. (2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis.
    Toxicol In Vitro. 28 (5): 896-908.

Further Reading

  1. Kadowaki, N. et al. (2000) Natural interferon alpha/beta-producing cells link innate and adaptive immunity.
    J Exp Med. 192 (2): 219-26.