Find out more about A.I.den - our SearchBot

CD14 antibody | TÜK4

100% Secure
Anti Human CD14 Antibody, clone TÜK4 thumbnail image 1 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 2 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 3 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 4 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 5 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 6 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 7 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 8 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 9 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 10 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 11 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 12 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 13 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 14 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 15 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 16 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 17 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 18 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 19 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 20 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 21 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 22 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 23 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 24 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 25 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 26 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 27 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 28 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 29 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 30 Anti Human CD14 Antibody, clone TÜK4 thumbnail image 31
Anti Human CD14 Antibody, clone TÜK4 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD63 (MCA2142F) and Amethyst Orange conjugated Mouse IgG2a isotype control (MCA929AMO). Figure B. FITC conjugated Mouse anti Human CD63 (MCA2142F) and Amethyst Orange conjugated Mouse anti Human CD14 (MCA1568AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD14 Antibody, clone TÜK4 gallery image 2
Figure A. APC conjugated Mouse anti Human CD63 (MCA2142APC) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. APC conjugated Mouse anti Human CD63 (MCA2142APC) and FITC conjugated Mouse anti Human CD14 (MCA1568F). All experiments performed on human blood gated on live, single mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
Anti Human CD14 Antibody, clone TÜK4 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD31 (MCA1738F) and Alexa Fluor647 conjugated Mouse IgG2a isotype contol. Figure B. FITC conjugated Mouse anti Human CD31 (MCA1738F) and Alexa Fluor647 conjugated Mouse CD14 (MCA1568A647). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A)
Anti Human CD14 Antibody, clone TÜK4 gallery image 4
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD9 (MCA469A488) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD9 (MCA469A488) and RPE conjugated Mouse anti Human CD14 (MCA1568PE). All experiments performed on red cell lysed human blood gated on myeloid cells. Data acquired on the ZE5™ cell analyzer
Anti Human CD14 Antibody, clone TÜK4 gallery image 5
Sandwich ELISA analysis of Human CD14 binding using Mouse anti CD14 antibody, clone MEM-18 (MCA2185) as a capture reagent and biotinylated Mouse anti Human CD14 antibody, clone Tük4 (MCA1568B) as a detection reagent with purified CD14 as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum samples diluted 1:200 and 1:400 are shown green and red respectively, while plasma samples also diluted 1:200 and 1:400 are blue and orange respectively.
Anti Human CD14 Antibody, clone TÜK4 gallery image 6
Published customer image:
Phycoerythrin conjugated Mouse anti Human CD14 antibody, clone Tük4 (MCA1568PE) used for the evaluation of CD14 expression on bovine monocytes by flow cytometry.
Image caption:
Gating strategy utilized for flow cytometric analysis of bovine peripheral blood leukocytes showing representative data from one animal. After gating on viable (propidium-iodide-negative) cells (A), cell doublets were excluded by SSC-A and SSC-H gating (B). Bovine mononuclear cells (MNC) and granulocytes (PMN) were gated based on their forward and side scatter characteristics and their percentages were calculated (C). Three-color immunofluorescence of bovine MNC with mAbs to CD172a, CD14 and CD16 defines three monocyte subsets in peripheral blood. Viable mononuclear cells, based on forward and side scatter characteristics, were gated on CD172a-positive cells (D). Dot plots of CD14 and CD16 expression display classical monocytes (CD14+CD16−, upper left), intermediate monocytes (CD14+CD16+, upper right) and nonclassical monocytes (CD14−CD16+, lower right) (E)

From: Pomeroy B, Sipka A, Hussen J, Eger M, Schukken Y, Schuberth HJ.
Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 2017 Feb 21;48(1):13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 7
Published customer image:Mouse anti Human CD14 antibody clone Tük4 (MCA1568) used to evaluate CD14 expression on canine monocytes by flow cytometry.
Image caption:
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 8
Published customer image:
Mouse anti Human CD14 antibody clone Tük4 (MCA1568) used to evaluate CD14 expression on canine monocytes by flow cytometry.
Image caption:
Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14−MHCII− cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14−MHCII− cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14−MHCII− cells is shown at 63× magnification.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 9
Published customer image:
Mouse anti Human CD14 antibody clone Tük4 (MCA1568) used to evaluate CD14 expression on canine monocytes by flow cytometry.
Image caption:
CD11b+CD14+MHCII− cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII− cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 10
Published customer image:
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.
Image caption:
Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 μm. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 μm. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 μm.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 11
Published customer image:
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in lymph nodes of adjuvant injected calves by immunohistochemistry.
Image caption:
Cellular recruitment to lymph nodes (LN) and peripheral blood. (A) LN cells were prepared for FCM analysis and gated on forward/side scatter (FSC/SSC) characteristics. Plots from one representative animal are presented. Panels illustrate the gating of lymphocytes, monocytes, and granulocytes as indicated and in the draining LN (left) and the contralateral LN (right), at 24 h post-injection. (B) Percentages of major immune cell populations in LNs, based on the gating strategy in A. Horizontal stacked bars show mean percentages of lymphocytes (gray), monocytes (dark gray), and granulocytes (black) of the total live cell population in non-injected animals (n = 6) and at different time points after adjuvant injection (n = 2–3). (C) IHC labeling of draining and contralateral LNs at 24 h post-injection with antibody against CD14. Different regions of the LN are indicated. (D) Cellular differential counts in peripheral blood. Horizontal stacked bars show mean absolute numbers (×109) of lymphocytes (gray), monocytes (dark gray), and granulocytes (black) at pre-injection and at different time points after adjuvant injection.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 12
Published customer image:
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in lymph nodes of adjuvant injected calves by immunofluorescence.
Image caption:
Distribution of monocytes in the LNs. Immunofluorescent labeling of LNs with antibodies against CD14 (green), CD21 (blue), and Ki67 (red). CD21 stains the LN follicles. (A) CD14+ cells were present in the capsule, subcapsular sinus, peri-trabecular sinus, and interfollicular areas of the draining LN at 24 h post-injection. (B) The contralateral LN was mainly devoid of CD14+ cells. Note the empty sub capsular sinus and trabecular sinus areas, as opposed to the infiltration in (A). (C) CD14+ cells were abundant in the capsule, but were decreased in numbers in the sinus and the cortex at 48 h post-injection. (D) CD14+ cells were present in the medulla of the LN at 48 h post-injection, and particularly around vessels. Follicle (f), interfollicular area (i), capsule (c), sinus area (s), vessel (v). Scale bars: 100 μm.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 13
Published customer image:
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes and Mouse anti Bovine CD205 (MCA1651GA) to identify dendritic cells in lymph nodes and subcutaneous tissue of adjuvant injected calves by immunofluorescence.
Image caption:
Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14+ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205+ cells were present. (B) A few CD14+ cells (arrow) were observed on the contralateral side. (C) CD14+ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205+ CD11c+ DCs were observed (arrow heads in insert). (D) CD14+ cells were sparsely present in the contralateral LN. CD205+ follicles were surrounded by CD11c single labeled and CD11c+ CD205+ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 14
Published customer image:
RPE-AlexaFluor647 tandem conjugated Mouse anti Human CD14 antibody, clone Tük4 (MCA1568P647) used to identify CD14 expressing mononuclear cells in peripheral blood samples by flow cytometery.
Image caption:
Clinical relevance of CD11b+CD14+S100A9+ MDSCs in lung adenocarcinoma harboring activating EGFR mutation
(A) Representative dot plots of PBMC of NSCLC patients (CA) and normal health donors (NC). Non-lymphocyte mononuclear cell was gated, and CD11b+CD14+ S100A9+ cells were analyzed by flow cytometry. (B) Ratio of CD11b+CD14+ S100A9+ cells in PBMC of health donors (NC, n = 7) and NSCLC patients (CA, n = 58). (C) Clinical response of EGFR-TKI treatment and peripheral blood CD11b+CD14+ S100A9+ MDSCs, CR+PR, n = 46; SD+ PD, n = 12. (D) CD11b+CD14+ S100A9+ difference between DCB and NCB groups in training cohort. (DCB, n = 19; NCB, n = 6, p = 0.0007) (E) CD11b+CD14+ S100A9+ difference between DCB and NCB groups in validation cohort. (DCB, n = 26; NCB, n = 7, p = 0.06) (F–H) Kaplain-Meier curve of PFS according to median percentage of CD11b+CD14+S100A9+ in training cohort, validation cohort and all cases. Dark line: CD11b+CD14+S100A9+ ≤18.9% in PBMC, dashed line: CD11b+CD14+S100A9+ >18.9%. All data express as mean ±SD, * p <0.05; CR: complete response, PR: partial response, SD: stable disease, PD: progress disease, DCB: durable clinical benefit, NDB: non-durable benefit.

From: Feng PH, Yu CT, Chen KY, Luo CS, Wu SM, Liu CY, Kuo LW, Chan YF, Chen TT, Chang CC, Lee CN, Chuang HC, Lin CF, Han CL, Lee WH, Lee KY.
S100A9(+) MDSC and TAM-mediated EGFR-TKI resistance in lung adenocarcinoma: the role of RELB.
Oncotarget. 2018 Jan 10;9(7):7631-43.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 15
Figure A. APC conjugated Mouse anti Human CD63 (MCA2142APC) and Alexa Fluor 488 conjugated Mouse IgG2a isotype control (MCA929A488). Figure B. APC conjugated Mouse anti Human CD63 (MCA2142APC) and Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 16
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A647) and SBV515 conjugated Mouse anti Human CD33 (MCA1271SBV515). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 17
Figure A. FITC conjugated Mouse anti Human CD63 (MCA2142F). Figure B. FITC conjugated Mouse anti Human CD63 (MCA2142F) and SBV515 conjugated Mouse anti Human CD14 (MCA1568SBV515). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 18
Published customer image:
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes and Mouse anti Bovine CD205 (MCA1651GA) to identify dendritic cells in lymph nodes and subcutaneous tissue of adjuvant injected calves by immunofluorescence.
Image caption:
Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14+ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205+ cells were present. (B) A few CD14+ cells (arrow) were observed on the contralateral side. (C) CD14+ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205+ CD11c+ DCs were observed (arrow heads in insert). (D) CD14+ cells were sparsely present in the contralateral LN. CD205+ follicles were surrounded by CD11c single labeled and CD11c+ CD205+ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.

From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Human CD14 Antibody, clone TÜK4 gallery image 19
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 670 conjugated Mouse anti Human CD33 (MCA1271SBV670). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 20
Figure A. FITC conjugated Mouse anti Human CD63 (MCA2142F). Figure B. FITC conjugated Mouse anti Human CD63 (MCA2142F) and StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 21
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 440 conjugated Mouse anti Human CD33 (MCA1271SBV440). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 22
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD14 (MCA1568A488) and StarBright Violet 610 conjugated Mouse anti Human CD33 (MCA1271SBV610). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 23
Figure A. FITC conjugated Mouse anti Human CD63 (MCA2142F). Figure B. FITC conjugated Mouse anti Human CD63 (MCA2142F) and StarBright Violet 610 conjugated Mouse anti Human CD14 (MCA1568SBV610). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 24
Figure A. FITC conjugated Mouse anti Human CD63 (MCA2142F). Figure B. FITC conjugated Mouse anti Human CD63 (MCA2142F) and StarBright Violet 670 conjugated Mouse anti Human CD14 (MCA1568SBV670). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 25
Figure A. Pacific Blue conjugated Mouse anti Human CD16 (MCA2537PB). Figure B. Pacific Blue conjugated Mouse anti Human CD16 (MCA2537PB) and StarBright Violet 570 conjugated Mouse anti Human CD14 (MCA1568SBV570). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 26
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD14 (MCA1568A647) and StarBright Violet 570 conjugated Mouse anti Human CD33 (MCA1271SBV570). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 27
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA2537A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD16 (MCA2537A647) and StarBright Violet 710 conjugated Mouse anti Human CD14 (MCA1568SBV710). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 28
Figure A. PE-Alexa Fluor 750 conjugated Mouse anti Human CD14 (MCA1568P750). Figure B. PE-Alexa Fluor 750 conjugated Mouse anti Human CD14 (MCA1568P750) and StarBright Violet 710 conjugated Mouse anti Human CD33 (MCA1271SBV710). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 29
Figure A. Pacific Blue conjugated Mouse anti Human CD16 (MCA2537PB). Figure B. Pacific Blue conjugated Mouse anti Human CD16 (MCA2537PB) and StarBright Violet 790 conjugated Mouse anti Human CD14 (MCA1568SBV790). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 30
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790). All experiments performed on red blood cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD14 Antibody, clone TÜK4 gallery image 31
Published customer image:
RPE conjugated Mouse anti Bovine CD14 antibody, clone Tük4 (MCA1568PE) used to identify monocyte populations in bovime blood samples by flow cytometry.
Image caption:
Changes in the monocyte subsets of PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify monocyte subset population. A dot plot depicting the three monocyte subsets in monocytes (CD172a+): classical CD14+CD16-, intermediate CD14+CD16+, and non-classical CD14-CD16+ monocytes. MT indicates moderate THI condition and HT indicates high THI condition

From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human CD14:Alexa Fluor® 488

Mouse anti Human CD14:Alexa Fluor® 647

Mouse anti Human CD14:Biotin

Mouse anti Human CD14:FITC

Mouse anti Human CD14:Low Endotoxin

Mouse anti Human CD14:Pacific Blue®

Mouse anti Human CD14

Mouse anti Human CD14:RPE

Mouse anti Human CD14:RPE-Alexa Fluor® 647

Mouse anti Human CD14:RPE-Alexa Fluor® 750

Mouse anti Human CD14:Alexa Fluor® 700

Mouse anti Human CD14:StarBright Blue 700

Mouse anti Human CD14:Amethyst Orange

Mouse anti Human CD14:StarBright Violet 440

Mouse anti Human CD14:StarBright Violet 515

Mouse anti Human CD14:StarBright Violet 610

Mouse anti Human CD14:StarBright Violet 670

Mouse anti Human CD14:StarBright Violet 570

Mouse anti Human CD14:StarBright Violet 710

Mouse anti Human CD14:StarBright Violet 790

Mouse anti Human CD14:StarBright Violet 475

Mouse anti Human CD14:StarBright UltraViolet 400

Mouse anti Human CD14:StarBright UltraViolet 665

Product Type
Monoclonal Antibody
Clone
TÜK4
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1568A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568A647T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1568B
Datasheet
F
SDS
0.1 mg loader

MCA1568F
Datasheet
F
SDS
0.1 mg loader

MCA1568FT
Datasheet
F
SDS
25 µg loader

MCA1568EL
Datasheet
E F FN WB
SDS
0.5 mg loader

MCA1568PB
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568PBT
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1568GA
Datasheet
E F IF
SDS
0.1 mg loader

MCA1568
Datasheet
E F IF WB
SDS
0.2 mg loader

MCA1568T
Datasheet
E F WB
SDS
25 µg loader

MCA1568PE
Datasheet
F
SDS
100 Tests loader

MCA1568PET
Datasheet
F
SDS
25 Tests loader

MCA1568P647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568P750
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568A700
Datasheet
F
SDS
100 Tests/1ml loader

MCA1568A700T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1568SBB700
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568AMO
Datasheet
F
SDS
0.1 mg loader

MCA1568SBV440
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV515
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV610
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV670
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV570
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV710
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV790
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBV475
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBUV400
Datasheet
F
SDS
100 Tests/0.5ml loader

MCA1568SBUV665
Datasheet
F
SDS
100 Tests/0.5ml loader

Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986).

CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.

Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Goat
Cat
Rabbit
Mink
Bovine
Pig
Sheep
Cynomolgus monkey
Llama
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Product Form
Purified IgG conjugated to StarBright Blue 700 - liquid
Product Form
Purified IgG conjugated to Amethyst Orange - liquid
Product Form
Purified IgG conjugated to StarBright Violet 440 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 515 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 610 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 670 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 570 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 710 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 790 - liquid
Product Form
Purified IgG conjugated to StarBright Violet 475 - liquid
Product Form
Purified IgG conjugated to StarBright UltraViolet 400 - liquid
Product Form
Purified IgG conjugated to StarBright UltraViolet 665 - liquid
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1ml distilled water
Pack Size: 25 Tests
Reconstitute with 0.25ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 1.0 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.2 mg, 25 µg
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Carrier Free
Yes
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Pack Size: 100 Tests
Prior to reconstitution store at +4oC.

After reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 25 Tests
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/2 Pack Size: 0.1 mg
1/10 Pack Size: 25 µg
ELISA
Flow Cytometry 1/100 1/200
Functional Assays
Western Blotting
Flow Cytometry Neat 1/5
ELISA
Flow Cytometry 1/50 1/200
Immunofluorescence
Western Blotting
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 488 MCA929A488 F 100 Tests/1ml loader
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control:Biotin MCA929B F 100 Tests loader
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control:Low Endotoxin MCA929EL F 0.5 mg loader
Mouse IgG2a Negative Control:Pacific Blue® MCA929PB F 100 Tests/1ml loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader
Mouse IgG2a Negative Control:RPE-Alexa Fluor® 647 MCA929P647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control:Alexa Fluor® 700 MCA929A700 F 100 Tests/1ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immuno-electron Microscopy

Product Specific References

References for CD14 antibody

  1. Schenk, M. et al. (2005) Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
    J Immunol. 174 (1): 517-24.
  2. Willett, B.J. et al. (2003) Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection.
    J Virol. 77 (1): 709-12.
  3. Soell, M. et al. (1995) Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release.
    J Immunol. 154 (2): 851-60.
  4. Bryan, S.A. et al. (2002) Responses of leukocytes to chemokines in whole blood and their antagonism by novel CC-chemokine receptor 3 antagonists.
    Am J Respir Crit Care Med. 165: 1602-9.
  5. Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
    Vet Pathol. 38: 512-8.
  6. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
    Vet Immunol Immunopathol. 51 (1-2): 89-99.
  7. Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
    Vet Immunol Immunopathol. 56 (1-2): 11-25.
  8. Xiong, W. et al. (2010) Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial.
    PLoS One. 5: e11074.
  9. Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
    Vet Immunol Immunopathol. 62 (3): 185-95.
  10. Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion.
    Immunol Lett. 88 (1): 27-30.
  11. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  12. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
    Vet Immunol Immunopathol. 39 (4): 461-6.
  13. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  14. Dalli J et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  15. Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin Vaccine Immunol. 16 (7): 1003-11.
  16. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  17. Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
    J Virol. 80: 7873-84.
  18. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  19. Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
    Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95.
  20. Lund, H. et al. (2016) Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
    Front Immunol. 7: 322.
  21. Krueger, L.A. et al. (2016) Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves.
    J Dairy Sci. 99 (11): 9040-50.
  22. Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
    Vet Immunol Immunopathol. 159 (1-2): 91-6.
  23. Schaut, R.G. et al. (2015) Bovine viral diarrhea virus type 2 in vivo infection modulates TLR4 responsiveness in differentiated myeloid cells which is associated with decreased MyD88 expression.
    Virus Res. 208: 44-55.
  24. Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
    J Diabetes Res. 2016: 3486727.
  25. Pomeroy, B. et al. (2017) Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
    Vet Res. 48 (1): 13.
  26. Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
    Vet Immunol Immunopathol. 181: 15-23.
  27. Martini, V. et al. (2018) Flow cytometry for feline lymphoma: a retrospective study regarding pre-analytical factors possibly affecting the quality of samples.
    J Feline Med Surg. 20 (6): 494-501.
  28. Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
    Vet Comp Oncol. 14 (4): 409-16.
  29. Feng, P.H. et al. (2018) S100A9+ MDSC and TAM-mediated EGFR-TKI resistance in lung adenocarcinoma: the role of RELB.
    Oncotarget. 9 (7): 7631-43.
  30. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  31. Penadés, M. et al. (2020) Early deviations in performance, metabolic and immunological indicators affect stayability in rabbit females.
    Animal. 14 (4): 780-9.
  32. Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
    Viruses. 12 (3)Mar 07 [Epub ahead of print].
  33. Mas, A. et al. (2020) A further investigation of the leishmaniosis outbreak in Madrid (Spain): low-infectivity phenotype of the Leishmania infantum BOS1FL1 isolate to establish infection in canine cells.
    Vet Immunol Immunopathol. 230: 110148.
  34. Tuohy, J.L. et al. (2020) Immune dysregulation and osteosarcoma: Staphylococcus aureus. downregulates TGF-β and heightens the inflammatory signature in human and canine macrophages suppressed by osteosarcoma.
    Vet Comp Oncol. 18 (1): 64-75.
  35. Sipka, A.S. et al. (2020) The effect of ex vivo. lipopolysaccharide stimulation and nutrient availability on transition cow innate immune cell AKT/mTOR pathway responsiveness.
    J Dairy Sci. 103 (2): 1956-1968.
  36. Lessard, M. et al. (2018) Piglet weight gain during the first two weeks of lactation influences the immune system development.
    Vet Immunol Immunopathol. 206: 25-34.
  37. Moncada-Saucedo, N.K. et al. (2019) A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold.
    Stem Cells Int. 2019: 9792369.
  38. Muñoz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
    Am J Pathol. 190 (6): 1188-210.
  39. Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
    Cell Stress Chaperones. 26 (4): 705-20.
  40. Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
    Vet Comp Oncol. 19 (3): 567-77.
  41. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.

Further Reading

  1. Simmons, D. L. et al. (1989) Monocyte antigen CD14 is a phospholipid anchored membrane protein.
    Blood. 73:284-9.
  2. Bazil, V. et al. (1986) Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
    Eur J Immunol. 16:1583-9.
  3. Jersmann, H.P. (2005) Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.
    Immunol Cell Biol. 83:462-7.
  4. Pugin, J. et al. (1994) CD14 is a pattern recognition receptor.
    Immunity.1:509-16.
  5. Dziarski, R. et al. (1998) Binding of bacterial peptidoglycan to CD14.
    J Biol Chem. 273:8680-90.
  6. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra


Search for Batch Specific Datasheets

Flow Cytometry Workbook - Plan and improve your experiments

Free guide to become an antibody expert

View more products with CD14 specificity