Cetuximab antibody | AbD19834

Human anti Cetuximab

Product Type
Monoclonal Antibody
Clone
AbD19834
Isotype
HuCAL Fab monovalent
Specificity
Cetuximab

Product Code Applications Pack Size List Price Your Price Qty
HCA220
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Human Anti-Cetuximab Antibody, clone AbD19834, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target, epidermal growth factor receptor (EGFR). It can be used in bioanalytical assays to measure the levels of cetuximab and biosimilar products in preclinical research and clinical trials.

Clone AbD19834 is recommended as the capture antibody in a pharmacokinetic (PK) bridging ELISA. When paired with another inhibitory antibody, HRP conjugated Anti-Cetuximab Antibody clone AbD19830_hIgG1 (HCA221P), as detection antibody, free drug can be measured. When paired with the non-inhibitory Anti-Cetuximab Antibody, clone AbD19376_hIgG1 (HCA228P), free and partially bound drug can be measured, which is equivalent to total drug when trough serum levels are above 4 μg/ml.

Cetuximab (brand name Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.

View a summary of all anti cetuximab antibodies.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Cetuximab.
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD = 0.5 nM by real time, label free molecular interaction analysis on immobilized cetuximab.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Erbitux® is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a coating antibody in PK bridging assays together with HRP-conjugated HCA221 or HCA228 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

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Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Cetuximab HCA221 E 0.1 mg loader
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Description Human anti Cetuximab
Human anti Cetuximab HCA228 E 0.1 mg loader
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Description Human anti Cetuximab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP

Further Reading

  1. Gomez, D. et al. (2013) Cetuximab therapy in the treatment of metastatic colorectal cancer: the future frontier?
    Int J Surg. 11 (7): 507-13.

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Cetuximab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"