Cetuximab Antibody | AbD19376_hIgG1

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Human anti cetuximab specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti cetuximab (clone AbD19376_hIgG1) at a concentration of 2 µg/ml and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti cetuximab specificity titration ELISA

A microtiter plate was coated over night with human IgG1/kappa, human IgG1/lambda, rituximab or cetuximab at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti cetuximab (clone AbD19376_hIgG1 in a monovalent format) titrated to the given concentrations in PBST+10% human serum, followed by Mouse anti histidine tag:HRP (MCA5995P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Cetuximab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti cetuximab (clone AbD19834, HCA220) at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of cetuximab. Detection was performed using HRP-conjugated Human anti cetuximab (clone AbD19376_hIgG1) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Cetuximab inhibition ELISA

A microtiter plate was coated over night with EGFR at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of cetuximab (CTX, 0.3 µg/ml) plus increasing concentrations of the non-inhibitory Human anti cetuximab (HCA228) was added. In parallel experiments, HCA228 was replaced with inhibitory Human anti cetuximab antibodies of varying affinity (HCA220, HCA221 or HCA223). For this assay, HCA221, HCA223 and HCA228 were used in their monovalent Fab format. Free cetuximab, still capable of binding to the EGFR-coated plate, was detected using Goat anti Human IgG F(ab')2:HRP (STAR126P)

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  • Human anti Cetuximab
  • Human anti Cetuximab:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-cetuximab antibody is a non-neutralizing anti-idiotypic IgG1 antibody; it is ideal for bioanalytical assays for cetuximab and biosimilars. It does not inhibit the binding of cetuximab to EGFR; it is suitable as a detection antibody in PK bridging ELISA with HCA220 to measure total drug.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD19376_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA228Edatasheet pdfdatasheet pdf0.1 mg
      HCA228
      HCA228PEdatasheet pdfdatasheet pdf0.1 mg
      HCA228P
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti cetuximab, clone AbD19376_hIgG1, is a non-inhibitory anti-idiotypic antibody that binds to cetuximab. This antibody recognizes an epitope outside the cetuximab binding site and so does not inhibit the binding of the drug to its target. This epitope is shared with other chimeric antibodies that are highly homologous to cetuximab including infliximab but excluding rituximab.

        Clone AbD19376_hIgG1 is a fully human antibody and may be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) levels in patient sera. In the HRP-conjugated format, clone AbD19376_IgG1 is suitable as a detection antibody in pharmacokinetic (PK) assays to measure total cetuximab levels (free drug, partially bound and fully bound). Anti cetuximab antibody HCA220 is recommended as the capture antibody.

        Cetuximab (brand name Erbitux®) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.

        View a summary of all anti cetuximab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Chimeric monoclonal antibody
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=39 nM by real time, label-free molecular interaction analysis on immobilized cetuximab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Erbitux®is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Erbitux®is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or when conjugated to HRP, as a detection antibody in a cetuximab bridging ELISA for PK assay development together with HCA220 as the capture reagent.
        Protocol: PK bridging ELISA to measure total drug
      • ELISA
        This product may be used in a direct ELISA or as a detection antibody in a cetuximab bridging ELISA for PK assay development together with HCA220 as the capture reagent.
        Protocol: PK bridging ELISA to measure total drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Cetuximab Antibody Formats

      Formats Clone Applications Sizes available
      Cetuximab Antibody : Purified AbD19376_hIgG1 E 0.1 mg
      Cetuximab Antibody : HRP AbD19376_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti CetuximabHCA2200.1 mgE
            HCA220
            Human anti CetuximabHCA2210.1 mgE
            HCA221
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti CetuximabHCA2200.1 mgE
            HCA220
            Human anti CetuximabHCA2210.1 mgE
            HCA221
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

                References

                Further Reading

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