Cetuximab antibody | AbD19834

100% Secure


Human anti Cetuximab

Anti-Cetuximab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development for cetuximab and biosimilars. It is suitable as a capture antibody in PK bridging ELISA format with HCA221 for an assay to detect free drug.

Product Type
Monoclonal Antibody
Clone
AbD19834
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA220
Datasheet
E
SDS
0.1 mg loader

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD = 0.5 nM by real time, label free molecular interaction analysis on immobilized cetuximab.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Erbitux® is a registered trademark of ImClone LLC, a wholly-owned subsidiary of Eli Lilly and Company.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Cetuximab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a coating antibody in PK bridging assays together with HRP-conjugated HCA221 or HCA228 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg loader
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Cetuximab HCA221 E 0.1 mg loader
Human anti Cetuximab HCA228 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader

Product Specific References

Further Reading

  1. Gomez, D. et al. (2013) Cetuximab therapy in the treatment of metastatic colorectal cancer: the future frontier?
    Int J Surg. 11 (7): 507-13.

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸


How to Use the Spectraviewer?

Watch the Tool Tutorial Video ▸

  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra