Bevacizumab antibody | AbD17976
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Bevacizumab Antibody clone AbD17976 (HCA182) was added at a concentration of 2 μg/ml in PBST. Detection was performed using Mouse Anti-Strep-Tag Classic:HRP (MCA2489P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with recombinant human VEGF protein at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of bevacizumab (0.3 μg/ml) plus increasing concentrations of Human Anti-Bevacizumab Antibody, clone AbD17976 (HCA182) in the monovalent Fab format was added. Free bevacizumab, still capable of binding to the human VEGF coated plate was detected using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP (MCA647P) in HISPEC Assay diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Bevacizumab Antibody, clone AbD17976 (HCA182) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of bevacizumab. Detection was performed by HRP conjugated Human Anti-Bevacizumab Antibody, clone AbD16748_hIgG1 (HCA184P) at a concentration of 4 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Human anti Bevacizumab
Anti-bevacizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of bevacizumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA with HCA184/HCA184P for detection.
- Product Type
- Monoclonal Antibody
- Clone
- AbD17976
- Isotype
- HuCAL Fab monovalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA182 | E | 0.1 mg |
|
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| HCA182G | E | 1 mg |
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Human Anti-Bevacizumab Antibody, clone AbD17976 is a paratope specific, recombinant, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug bevacizumab and inhibits the binding of the drug to its target, vascular endothelial growth factor A (VEGF-A). The antibody can be used to measure the levels of free bevacizumab and biosimilar products using bioanalytical assays.
A pair of anti-bevacizumab antibodies can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Bevacizumab Antibody in full immunoglobulin format, clone AbD16748_hIgG1 (HCA184P) as the detection antibody.
Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.
View a summary of all Anti-Bevacizumab Antibodies.
A pair of anti-bevacizumab antibodies can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Bevacizumab Antibody in full immunoglobulin format, clone AbD16748_hIgG1 (HCA184P) as the detection antibody.
Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.
View a summary of all Anti-Bevacizumab Antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Bevacizumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
- Approx. Protein Concentrations
- Pack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Avastin is a trademark of Genentech, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Bevacizumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA184P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E WB | 0.1 mg |
|
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| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
|
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| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
|
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| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Bevacizumab | HCA184 | E | 0.1 mg |
|
|
| Human anti Bevacizumab | HCA185 | E | 0.1 mg |
|
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody |
Product Specific References
References for Bevacizumab antibody
-
McFarland, T.J. et al. (2015) BEVACIZUMAB LEVELS IN BREAST MILK AFTER LONG-TERM INTRAVITREAL INJECTIONS.
Retina. 35 (8): 1670-3. -
Iwamoto, N. et al. (2018) Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Anal Biochem. 540-541: 30-7.
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