Strep-Tag Classic antibody | Strep-tag II

Mouse anti Strep-Tag Classic:HRP

Product Type
Monoclonal Antibody
Clone
Strep-tag II
Isotype
IgG1
Specificity
Strep-Tag Classic

Product Code Applications Pack Size List Price Your Price Qty
MCA2489P
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E WB 75 µl loader
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Mouse anti Strep-Tag Classic antibody, clone Strep-tag II , also known as StrepMAB-Classic, recognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications.

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% ProClin 300
0.01% Gentamycin Sulphate
0.5% Bovine Serum Albumin
Stabilizing agents
Immunogen
Strep-tag II peptide (WSHPQFEK) conjugated to Keyhole Limpet Hemocyanin.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
Sold under license of IBA.
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/10,000
Western Blotting 1/2000 1/32,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for Strep-Tag Classic antibody

  1. Renzi F et al. (2015) Glycan-Foraging Systems Reveal the Adaptation of Capnocytophaga canimorsus to the Dog Mouth.
    MBio. 6 (2): .pii: e02507-14.
  2. Gordon, C.A. et al. (2015) NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.
    Prostate. 75 (5): 517-26.
  3. Mavrakis, M. et al. (2016) Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
    Methods Cell Biol. 136: 199-220.
  4. Oda, S. et al. (2015) Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression.
    J Virol. 90 (4): 1839-48.
  5. Renzi, F. et al. (2015) Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth.
    MBio. 6 (2): e02507.

RRID
AB_609796