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Strep-Tag Classic antibody | Strep-tag II

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Anti Strep-Tag Classic Antibody, clone Strep-tag II gallery image 1
HCA182 specificity ELISA using various antigens (A: Human Serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Ustekinumab, E: Infliximab, F: Adalimumab, G: Alemtuzumab and H: Bevacizumab) as coating components followed by Human anti Avastin®(HCA182) and HRP conjugated Mouse anti Strep-tag (MCA2489P) as detection reagent.

Mouse anti Strep-Tag Classic:HRP

Mouse anti Strep-Tag Classic

Product Type
Monoclonal Antibody
Clone
Strep-tag II
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2489P
Datasheet
E WB
SDS
75 µl loader

MCA2489
Datasheet
E WB
SDS
0.1 mg loader

Mouse anti Strep-Tag Classic antibody, clone Strep-tag II , also known as StrepMAB-Classic, recognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications.

Product Details

Target Species
Synthetic Peptide
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml phosphate buffered saline.
The addition of 0.09% sodium azide is recommended for long term storage.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Ammonium bicarbonate
1% Sucrose
Preservative Stabilisers
0.05% Proclin
0.01% Gentamycin Sulphate
0.5% Bovine Serum Albumin
Stabilizing agents
Preservative Stabilisers
None present
Immunogen
Strep-tag II peptide (WSHPQFEK) conjugated to Keyhole Limpet Hemocyanin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of IBA
Acknowledgements
Sold under license of IBA
Regulatory
For research purposes only

Applications of Strep-Tag Classic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/10,000
Western Blotting 1/2000 1/32,000
ELISA
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for Strep-Tag Classic antibody

  1. Renzi F et al. (2015) Glycan-Foraging Systems Reveal the Adaptation of Capnocytophaga canimorsus to the Dog Mouth.
    MBio. 6 (2): .pii: e02507-14.
  2. Gordon, C.A. et al. (2015) NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.
    Prostate. 75 (5): 517-26.
  3. Mavrakis, M. et al. (2016) Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
    Methods Cell Biol. 136: 199-220.
  4. Oda, S. et al. (2015) Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression.
    J Virol. 90 (4): 1839-48.
  5. Renzi, F. et al. (2015) Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth.
    MBio. 6 (2): e02507.
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