Strep-Tag Classic antibody | Strep-tag II

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Anti Strep-Tag Classic Antibody, clone Strep-tag II gallery image 1 — HCA182 specificity ELISA using various antigens (A: Human Serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Ustekinumab, E: Infliximab, F: Adalimumab, G: Alemtuzumab and H: Bevacizumab) as coating components followed by Human anti Avastin^®(HCA182) and HRP conjugated Mouse anti Strep-tag (**MCA2489P**) as detection reagent.

Mouse anti Strep-Tag Classic:HRP

Mouse anti Strep-Tag Classic

Product Type: Monoclonal Antibody
Clone: Strep-tag II
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2489P	E WB			75 µl
MCA2489	E WB			0.1 mg

Mouse anti Strep-Tag Classic antibody, clone Strep-tag II , also known as StrepMAB-Classic, recognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications.

Product Details

Target Species: Synthetic Peptide
Product Form: Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml phosphate buffered saline.
The addition of 0.09% sodium azide is recommended for long term storage.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Ammonium bicarbonate
1% Sucrose
Preservative Stabilisers: 0.05% Proclin
0.01% Gentamycin Sulphate
0.5% Bovine Serum Albumin
Stabilizing agents
Preservative Stabilisers: None present
Immunogen: Strep-tag II peptide (WSHPQFEK) conjugated to Keyhole Limpet Hemocyanin.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at -20^oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

Acknowledgements: Sold under license of IBA
Acknowledgements: Sold under license of IBA
Regulatory: For research purposes only

Applications of Strep-Tag Classic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/5000	1/10,000
Western Blotting	1/2000	1/32,000
ELISA
Western Blotting	1/1000	1/5000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for Strep-Tag Classic antibody

Renzi F et al. (2015) Glycan-Foraging Systems Reveal the Adaptation of Capnocytophaga canimorsus to the Dog Mouth.
MBio. 6 (2): .pii: e02507-14.
Gordon, C.A. et al. (2015) NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.
Prostate. 75 (5): 517-26.
Mavrakis, M. et al. (2016) Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
Methods Cell Biol. 136: 199-220.
Oda, S. et al. (2015) Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression.
J Virol. 90 (4): 1839-48.
Renzi, F. et al. (2015) Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth.
MBio. 6 (2): e02507.

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