Strep-Tag Classic antibody | Strep-tag II
Mouse anti Strep-Tag Classic antibody, clone Strep-tag II , also known as StrepMAB-Classic, recognizes Strep-tag II, a widely used tag in protein expression applications. This antibody recognizes both-C- and N-terminal Strep-tag II and is especially suited to Western blot applications.
Product Details
- Target Species
- Synthetic Peptide
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml phosphate buffered saline.
The addition of 0.09% sodium azide is recommended for long term storage. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Ammonium bicarbonate
1% Sucrose - Preservative Stabilisers
- 0.05% Proclin
0.01% Gentamycin Sulphate
0.5% Bovine Serum Albumin
Stabilizing agents - Preservative Stabilisers
- None present
- Immunogen
- Strep-tag II peptide (WSHPQFEK) conjugated to Keyhole Limpet Hemocyanin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of IBA
- Acknowledgements
- Sold under license of IBA
- Regulatory
- For research purposes only
Applications of Strep-Tag Classic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/5000 | 1/10,000 | |
| Western Blotting | 1/2000 | 1/32,000 | |
| ELISA | |||
| Western Blotting | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Strep-Tag Classic antibody
-
Renzi F et al. (2015) Glycan-Foraging Systems Reveal the Adaptation of Capnocytophaga canimorsus to the Dog Mouth.
MBio. 6 (2): .pii: e02507-14. -
Gordon, C.A. et al. (2015) NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells.
Prostate. 75 (5): 517-26. -
Mavrakis, M. et al. (2016) Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
Methods Cell Biol. 136: 199-220. -
Oda, S. et al. (2015) Crystal Structure of Marburg Virus VP40 Reveals a Broad, Basic Patch for Matrix Assembly and a Requirement of the N-Terminal Domain for Immunosuppression.
J Virol. 90 (4): 1839-48. -
Renzi, F. et al. (2015) Glycan-foraging systems reveal the adaptation of Capnocytophaga canimorsus to the dog mouth.
MBio. 6 (2): e02507.
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