V5-Tag antibody | SV5-Pk1
Mouse anti V5-Tag:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- SV5-Pk1
- Isotype
- IgG2a
- Specificity
- V5-Tag
Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005). |
Our V5-Tag (SV5-Pk1) Antibody has been referenced in >215 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Paramyxovirus Simian-Virus 5 (SV5)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is manufactured under an exclusive license from the University of St. Andrews, UK.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/250 | 1/2500 | |
Immunohistology - Frozen | |||
Western Blotting | 1/1000 | 1/5000 |
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for V5-Tag antibody
-
Southern, J.A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.
J Gen Virol. 72 ( Pt 7): 1551-7. -
Randall, R.E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming.
Vaccine. 12 (4): 351-8. -
Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal.
EMBO J. 13 (22): 5410-20. -
Rodriguez, M.S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein.
Mol Cell Biol. 15 (5): 2413-9. -
Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65.
Mol Cell Biol. 15 (4): 2166-72. -
Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding.
J Virol Methods. 53 (1): 149-56. -
Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
J Immunol Methods. 224 (1-2): 141-50. -
Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells.
J Virol. 75: 3363-70.
View The Latest Product References
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Sanchez Garcia, J. et al. (2004) The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.
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Zhao, C. et al. (2005) Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways.
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- Synonyms
- PK-TAG
- RRID
- AB_324248
- UniProt
- P11207
- GO Terms
- GO:0003723 RNA binding
- GO:0046872 metal ion binding
- GO:0016070 RNA metabolic process
- GO:0030683 evasion by virus of host immune response
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