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V5-Tag antibody | SV5-Pk1

Mouse anti V5-Tag:Biotin

Product Type
Monoclonal Antibody
Clone
SV5-Pk1
Isotype
IgG2a
Specificity
V5-Tag

Product Code Applications Pack Size List Price Your Price Qty
MCA1360B
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SDS Safety Datasheet SDS
C E WB 0.1 mg loader
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Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
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Our V5-Tag (SV5-Pk1) Antibody has been referenced in >215 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Viral
Product Form
Purified IgG conjugated to biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Paramyxovirus Simian-Virus 5 (SV5)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is manufactured under an exclusive license from the University of St. Andrews, UK.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/250 1/2500
Immunohistology - Frozen
Western Blotting 1/1000 1/5000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for V5-Tag antibody

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  9. View The Latest Product References
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Immunofluorescence

Immunoprecipitation

Western Blotting

Synonyms
PK-TAG
RRID
AB_324248
UniProt
P11207
GO Terms
GO:0003723 RNA binding
GO:0046872 metal ion binding
GO:0016070 RNA metabolic process
GO:0030683 evasion by virus of host immune response

MCA1360B

151579 158832 159420 164274 164559

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