Bevacizumab antibody | AbD17976

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Human anti Bevacizumab

Anti-bevacizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of bevacizumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA with HCA184/HCA184P for detection.

Product Type
Monoclonal Antibody
Clone
AbD17976
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA182 E 0.1 mg
HCA182G E 1 mg
Human Anti-Bevacizumab Antibody, clone AbD17976 is a paratope specific, recombinant, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug bevacizumab and inhibits the binding of the drug to its target, vascular endothelial growth factor A (VEGF-A). The antibody can be used to measure the levels of free bevacizumab and biosimilar products using bioanalytical assays.

A pair of anti-bevacizumab antibodies can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Bevacizumab Antibody in full immunoglobulin format, clone AbD16748_hIgG1 (HCA184P) as the detection antibody.

Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.

View a summary of all Anti-Bevacizumab Antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Bevacizumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

Avastin is a trademark of Genentech, Inc.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Bevacizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA184P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti V5-Tag:Alk. Phos. MCA1360A 0.1 mg E WB
Mouse anti V5-Tag:Biotin MCA1360B 0.1 mg C E WB
Mouse anti V5-Tag:HRP MCA1360P 0.1 mg E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Bevacizumab HCA184 0.1 mg E
Human anti Bevacizumab HCA185 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ

Product Specific References

References for Bevacizumab antibody

  1. McFarland, T.J. et al. (2015) BEVACIZUMAB LEVELS IN BREAST MILK AFTER LONG-TERM INTRAVITREAL INJECTIONS.
    Retina. 35 (8): 1670-3.