Alemtuzumab antibody | AbD19189
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E F ResetHuman anti Alemtuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD19189
- Isotype
- HuCAL Fab monovalent
- Specificity
- Alemtuzumab
Antibody Quality and 
Unrivaled 
| Human anti Alemtuzumab antibody, clone AbD19189 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis. Human anti Alemtuzumab antibody, clone AbD19189 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD19189_hIgG1 or clone AbD16942. Clone AbD19189 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells. View a summary of all anti-alemtuzumab antibodies. |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Alemtuzumab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a bridging ELISA together with HCA175P or HCA199 as the detection reagent when conjugated to HRP.
Protocol: PK bridging ELISA to measure free drug
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Rabbit anti DYKDDDDK Tag | AHP1074GA | E WB | 0.1 mg |
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| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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| Description | Mouse anti Histidine Tag:Alk.Phos. | ||||||
| Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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| Mouse anti Histidine Tag | MCA1396GA | E IP WB | 0.1 mg |
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| Description | Mouse anti Histidine Tag | ||||||
| Rat anti DYKDDDDK Tag | MCA4764GA | E F IF IP P WB | 0.1 mg |
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| Description | Rat anti DYKDDDDK Tag | ||||||
| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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| Description | Rat anti DYKDDDDK Tag:HRP | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
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| Description | Human anti Alemtuzumab | ||||||
| Human anti Alemtuzumab | HCA175 | E | 0.1 mg |
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| Description | Human anti Alemtuzumab | ||||||
| Human anti Alemtuzumab:HRP | HCA175P | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Alemtuzumab:HRP | ||||||
| Human anti Alemtuzumab | HCA199 | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Alemtuzumab | ||||||
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Alemtuzumab specificity
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