Alemtuzumab antibody | AbD19189
Human anti Alemtuzumab
Anti-alemtuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development to measure levels of alemtuzumab or biosimilars. It is recommended as capture antibody in PK bridging ELISA with HCA175/HCA175P.
- Product Type
- Monoclonal Antibody
- Clone
- AbD19189
- Isotype
- HuCAL Fab monovalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA198 | E | 0.1 mg |
|
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Alemtuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a bridging ELISA together with HCA175P or HCA199 as the detection reagent when conjugated to HRP.
Protocol: PK bridging ELISA to measure free drug
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti DYKDDDDK Tag | AHP1074GA | E WB | 0.1 mg |
|
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| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
|
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| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
|
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| Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
|
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| Mouse anti Histidine Tag | MCA1396GA | E IP WB | 0.1 mg |
|
|
| Rat anti DYKDDDDK Tag | MCA4764GA | E F IF IP P WB | 0.1 mg |
|
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| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
|
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| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Alemtuzumab | HCA174 | E | 0.1 mg |
|
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| Human anti Alemtuzumab | HCA175 | E | 0.1 mg |
|
|
| Human anti Alemtuzumab:HRP | HCA175P | E | 0.1 mg |
|
|
| Human anti Alemtuzumab | HCA199 | E | 0.1 mg |
|
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