Alemtuzumab antibody | AbD16728

Human anti Alemtuzumab

Product Type
Monoclonal Antibody
Clone
AbD16728
Isotype
HuCAL Fab monovalent
Specificity
Alemtuzumab

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HCA174
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Human anti Alemtuzumab antibody, clone AbD16728 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

Human anti Alemtuzumab antibody, clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.

This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P).

Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

View a summary of all anti-alemtuzumab antibodies.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
Preparation
StrepTactin affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Alemtuzumab.
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=1.6 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA175P as the detection reagent. Protocol: PK bridging ELISA to measure free drug

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Description Mouse anti V5-Tag:Biotin
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Description Mouse anti V5-Tag:HRP
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Description Mouse anti Strep-Tag Immo
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Description Mouse anti Strep-Tag Classic
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Description Mouse anti Strep-Tag Classic:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Alemtuzumab:HRP HCA175P E 0.1 mg loader
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Description Human anti Alemtuzumab:HRP
Rat anti Human CD52 MCA1642 C CT E F IP P RE WB 0.2 mg loader
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Description Rat anti Human CD52

RRID
AB_11152765
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"