Alemtuzumab Antibody | AbD19189_hIgG1

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Alemtuzumab Antibody | AbD19189_hIgG1 gallery image 1

HCA199 specificity ELISA using various antigens as coating components followed by HRP conjugated Human anti Alemtuzumab (HCA199) as the detection reagent

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HCA199 ADA (anti-drug antibodies) assay. Alemtuzumab was used as the coating antigen with Human anti Alemtuzumab spiked in human serum as the antigen followed by HRP conjugated Alemtuzumab as the detection reagent

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Binding of FITC conjugated Alemtuzumab to human lymphocytes (blue unshaded histogram) and subsequent inhibition of binding (red shaded histogram) in the presence of a 5 fold molar excess of HCA199 (A) or the corresponding negative control (B)

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  • Human anti Alemtuzumab
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    Anti-alemtuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human IgG1 format, suitable for bioanalytical method development. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD19189_hIgG1
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA199Edatasheet pdfdatasheet pdf0.1 mg
      HCA199
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti Alemtuzumab antibody, clone AbD19189_hIgG1, recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

        Clone AbD19189_hIgG1 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.

        This antibody, when conjugated to HRP, may be used in a bridging ELISA in conjunction with clone AbD19189 or clone AbD16942.

        Clone AbD19189_hIgG1 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

        View a summary of all anti-alemtuzumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A.
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Alemtuzumab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a detection antibody in a bridging ELISA when conjugated to HRP together with HCA198 as the capture reagent.

        A Lynx HRP rapid conjugation kit may be used to conjugate this antibody to HRP.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay.
        Protocol: ADA bridging ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Alemtuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Alemtuzumab Antibody : Purified AbD19189_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Alemtuzumab:HRPHCA175P0.1 mgE
            HCA175P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti AlemtuzumabHCA1740.1 mgE
            HCA174
            Human anti AlemtuzumabHCA1750.1 mgE
            HCA175
            Human anti AlemtuzumabHCA1980.1 mgE
            HCA198
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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