IL-2 Antibody

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IL-2 Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-2 (AHP1032) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0)

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IL-2 Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-2 (AHP1032) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0)

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IL-2 Antibody gallery image 3

Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 (MCA1553) as a capture reagent and biotinylated Rabbit anti Human IL-2 (AHP1032B<) as a detection reagent with recombinant human IL-2 (PHP042) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected

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  • Rabbit anti Human Interleukin-2:Biotin
  • Rabbit anti Human Interleukin-2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1032E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1032
    AHP1032BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP1032B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Interleukin-2 antibody recognizes human IL-2 (Interleukin-2), otherwise known as T-cell growth factor, a 133 amino acid glycoprotein and potent immunoregulatory cytokine produced by antigen or mitogen stimulated T cells.

      IL-2 is a multi-functional cytokine which signals through the IL-2 receptor complex (IL-2R), and is principally involved in the stimulation of the T cell proliferative response, promoting T cell clonal expansion and the expansion of T memory cells expressing low levels of high-affinity IL-2R.

      IL-2 also stimulates the growth/differentiation of B cells, monocytes, NK cells, macrophages and lymphokine activated killer cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to human IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50ug/ml after reconstituiton.
      IgG concentration 1.0mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      growth factor activity
      immune response
      positive regulation of B cell proliferation
      cell-cell signaling
      cytokine activity
      T cell differentiation
      positive regulation of interleukin-17 production
      kinase activator activity
      anti-apoptosis
      cell adhesion
      extracellular space
      positive regulation of tissue remodeling
      negative regulation of B cell apoptosis
      interleukin-2 receptor binding
      natural killer cell activation
      positive regulation of activated T cell proliferation
      positive regulation of cell growth
      positive regulation of inflammatory response
      positive regulation of tyrosine phosphorylation of Stat5 protein
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.120.16ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human IL-2 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-2 antibody (AHP1032) and recombinant human IL-2 (PHP042) as the standard.
    • ELISA
      This purified human IL-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-2 antibody (AHP1032B) as the detection reagent and recombinant human IL-2 (PHP042) as the standard
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-2 Antibody Formats

    Formats Applications Sizes available
    IL-2 Antibody : Purified E, FN, P *, WB 0.1 mg
    IL-2 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-2AHP10320.1 mgE, FN, P *, WB
          AHP1032
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human Interleukin-2:BiotinAHP1032B50 µgE, WB
          AHP1032B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-2PHP042A10 µgE, FN, WB
            PHP042A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Human Interleukin-2PHP042A10 µgE, FN, WB
            PHP042A

            Histology Controls

              Further Reading

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