IL-2

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IL-2 gallery image 1

Sandwich ELISA analysis of human interleukin-2 using Rat anti Human IL-2 (MCA1553) as a capture reagent and biotinylated Rabbit anti Human IL-2 (AHP1032B) as a detection reagent with recombinant human IL-2 (PHP042) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected

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Published customer image:
Stimulation of human CD4+ve cells with recombinant human IL-2 prior to evaluation of phosphorylated STAT5 by flow cytometry.
Image caption:
a Analysis of JAK3 protein expression in B-cell lines of a healthy control (1), father of the patients (2), patient 1 (3), patient 2 (4) and a ?c-deficient SCID patient (5). Examination of GAPDH protein expression served as a loading control. b Analysis of JAK3 signaling function in B-cell lines of a healthy control, patient 1 (II-1), her father (I-1) and a ?c-deficient SCID patient after stimulation with IL-4 and IL-21. Numbers in the top left and right corners of each histogram in panel B indicate the Mean Fluorescence Intensity (MFI) values of unstimulated and cytokine-stimulated cells, respectively. c Analysis of JAK3 signaling function in CD4+ peripheral blood T-cells of a healthy control, patient 1 (II-1) and patient 2 (II-2) after stimulation with IL-2. Histogram overlays represent intracellular levels of phosphorylated STAT5 in CD4+ T-cells without stimulation or after stimulation with IL-2 or IL-6. Numbers in the left top corner and middle part of each histogram indicate percentages of cells with a positive staining for pSTAT5 following stimulation with IL-2 or IL-6, respectively, while numbers in right corner constitute percentages of pSTAT5-positive unstimulated control cells.

From: Ban SA, Salzer E, Eibl MM, Linder A, Geier CB, Santos-Valente E, Garncarz W, Lion T, Ott R, Seelbach C, Boztug K, Wolf HM. Combined immunodeficiency evolving into predominant CD4+ lymphopenia caused by somatic chimerism in JAK3.
J Clin Immunol. 2014 Nov;34(8):941-53.

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  • Recombinant Human Interleukin-2
  • Recombinant Human Interleukin-2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP042AE, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP042A
    PHP042E, FN, WBdatasheet pdfdatasheet pdf50 µg
    PHP042
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Recombinant human interleukin 2 is an Escherichia coli-derived recombinant protein corresponding to full-length mature human interleukin-2, a secreted peptide required for T-cell proliferation. This preparation has an ED50 of 0.1ng/ml as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      Rhesus Monkeyyes
      Dolphinyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified protein produced in E. coli - lyophilised
      Purified protein produced in E. coli - lyophilised
    • Reconstitution
      Pack Size: 10 µgReconstitute with 0.1ml 0.1M acetic acid
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 50 µgReconstitute with 0.5 ml 0.1M acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 10 µgReconstitute with 0.1ml 0.1M acetic acid
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 50 µgReconstitute with 0.5 ml 0.1M acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
      None present
    • Activity
      1 x 107 units/mg
    • Purity
      >98% by SDS PAGE and HPLC analysis
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      Pack Size: 10 µgTotal protein concentration 0.1mg/ml after reconstitution.
      Pack Size: 50 µgTotal protein concentration 0.1 mg/ml after reconstitution
      Pack Size: 10 µgTotal protein concentration 0.1mg/ml after reconstitution.
      Pack Size: 50 µgTotal protein concentration 0.1 mg/ml after reconstitution
    • Protein Molecular Weight
      15.4kDa (134 Amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      10mM Sodium citrate.
      10mM Sodium citrate.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
      3 months from date of reconstitution.
    • GO Terms
      cytokine activity
      positive regulation of tyrosine phosphorylation of Stat5 protein
      negative regulation of B cell apoptosis
      anti-apoptosis
      positive regulation of activated T cell proliferation
      T cell differentiation
      cell adhesion
      interleukin-2 receptor binding
      kinase activator activity
      positive regulation of inflammatory response
      immune response
      growth factor activity
      cell-cell signaling
      positive regulation of interleukin-17 production
      positive regulation of cell growth
      positive regulation of B cell proliferation
      positive regulation of tissue remodeling
      natural killer cell activation
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.1ng/mlPack Size: 50 µg
      0.1Pack Size: 10 µg
      2.0ng/ml
      Western Blotting1.53.0ng/lane
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays
      0.1ng/mlPack Size: 50 µg
      0.1Pack Size: 10 µg
      2.0ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a standard in ELISA applications with either AHP1032 or AHP1032B.
    • ELISA
      This product may be used as a standard for ELISA applications with Rat anti Human IL-2 antibody, clone MQ1-17H12 as a capture reagent and biotinylated Rabbit anti Human IL-2 antibody for detection.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This product may be used as a positive control in western blotting applications with either AHP1032 or AHP1032B.
    • Western Blotting
      Recombinant human IL-2 may be used as a positive control for Western Blotting applications using Rabbit anti Human IL-2 antibodies for detection
    • Instructions For Use
    • Instructions For Use

    Additional IL-2 Formats

    Formats Applications Sizes available
    IL-2 : Rec. Protein E, FN, WB 50 µg | 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human Interleukin-2:BiotinAHP1032B50 µgE, WB
          AHP1032B
          Rabbit anti Human Interleukin-2AHP10320.1 mgE, FN, P *, WB
          AHP1032

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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