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A personal, no obligation quotation for a custom monoclonal antibody generation project
Tel: +49 (0) 89 80 90 95 45
Fax: +49 (0) 89 80 90 95 50
Office: Zeppelinstr. 4, 82178 Puchheim, Germany
We understand that an antibody for use as a critical reagent in clinical studies, or as a raw material in a regulated IVD test, needs to be well characterized and of consistent quality.
Our extended quality control (QC) services are available for recombinant antibodies made as part of a custom HuCAL® project. They provide data to enable lot-to-lot comparison, and assist selection of the most appropriate antibody clone for your assay requirements.
Testing of each newly produced lot of an antibody in comparison with the reference material, and delivery of data showing degree of deviation from the reference lot; provides knowledge of the lot-to-lot consistency of the clone when ordering over months or years.
Tests to provide characterization data for an antibody and enable comparison between different clones of the same specificity, assisting selection of the best clone for the assay.
Fig. 1. Specificity titration ELISA for three lots of
Anti-Ipilimumab Antibody, clone AbD34433 (HCA330).
Fig. 2. An anti-drug antibody (ADA) bridging ELISA for five lots of Anti-Ipilimumab Antibody, clone AbD34429ia (HCA329); reference lot in green.
The package of services is available for Fab antibodies and purified full length immunoglobulin formats for all IgG isotypes, IgA, and IgE. Freeze-thaw testing and a shorter accelerated stability study are also available separately as individual assays.
Fig. 3. An ADA bridging ELISA for Anti-Ustekinumab Antibody AbD17827_hIgG1 (HCA210), after the indicated freeze-thaw cycles. The data show the antibody withstands a minimum of ten freeze-thaw cycles without any loss of activity.
Fig. 4. Accelerated stability testing of one lot of one antibody at three temperatures over four weeks comparison with control stored at -80°C. ADA bridging ELISA for Anti-Ustekinumab Antibody AbD17827_hIgG1 (HCA210); EC50 values were calculated from the nonlinear curve fits.
Fig. 5. Thermal stability of Anti-Ustekinumab Antibody clone AbD17827_hIgG1 (HCA210). A350/330 nm ratiometric measurement of the fluorescent signal plotted against temperature (upper curve) and determination of Tonset and Tm of the protein (lower curve); Tonset, temperature at which the protein starts to unfold; Tm, unfolding transition temperature, the point at which 50% of the protein is unfolded.
Learn how to secure high quality, reproducible and
sustainable critical antibody reagents early in the