Ipilimumab antibody | AbD34429ia





Human anti Ipilimumab:HRP
Human Anti-Ipilimumab Antibody is a recombinant, inhibitory anti-idiotypic antibody in IgG1 format for use in bioanalytical assays. It is labeled with HRP, and is recommended as the detection antibody in a PK bridging ELISA with HCA330 as capture antibody.
Human anti Ipilimumab
Human Anti-Ipilimumab Antibody is a recombinant, inhibitory anti-idiotypic antibody in IgG1 format for use in bioanalytical assays. It can be used as detection antibody in a PK bridging ELISA with HCA330, and as a positive control in an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD34429ia
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
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HCA329P | E | 0.1 mg |
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HCA329 | E | 0.1 mg |
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A pair of anti-ipilimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody is recommended as the detection antibody, paired with an anti-ipilimumab antibody in monovalent Fab format, clone AbD34433 (HCA330), as the capture antibody.
Clone AbD34429ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 1 nM, and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA327 has affinity 16 nM and HCA328 has affinity 0.3 nM.
Ipilimumab (Yervoy) is a human IgG1/kappa antibody developed from a transgenic mouse. It has been approved by the FDA for the treatment of metastatic melanoma, renal cell carcinoma, and in combination with nivolumab for the treatment of previously treated microsatellite instability-high/deficient mismatch repair (MSI-H/dMMR) metastatic colorectal cancer. Ipilimumab activates the immune system by targeting CTLA-4, a protein receptor that downregulates the immune system. The action of cytotoxic T lymphocytes (CTLs) to recognize and destroy cancer cells is subject to an inhibitory mechanism that interrupts this destruction. Ipilimumab turns off this inhibitory mechanism and allows CTLs to function.
View a summary of all anti-ipilimumab antibodies
Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is conjugated to horseradish peroxidase (HRP) and supplied as a liquid.
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Ipilimumab
- Affinity
- The monovalent intrinsic affinity of AbD34429ia was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized ipilimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70°C.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Yervoy is a trademark of Bristol-Myers Squibb Company. - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Yervoy is a trademark of Bristol-Myers Squibb Company. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Ipilimumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD34429ia can be used in a direct ELISA or as the detection antibody for ipilimumab in a bridging ELISA together with HCA330 as the capture reagent.
Protocol: PK bridging ELISA - ELISA
- Clone AbD34429ia can be used in an indirect ELISA or as a detection antibody for ipilimumab in a bridging ELISA together with HCA330 as the capture reagent. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA
Protocol: PK bridging ELISA
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Ipilimumab | HCA327 | E | 0.1 mg |
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Human anti Ipilimumab | HCA328 | E | 0.1 mg |
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Human anti Ipilimumab | HCA329 | E | 0.1 mg |
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Human anti Ipilimumab | HCA330 | E | 0.1 mg |
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Human anti Ipilimumab (Drug/Target Complex) | HCA331 | E | 0.1 mg |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Ipilimumab | HCA327 | E | 0.1 mg |
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Human anti Ipilimumab | HCA328 | E | 0.1 mg |
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Human anti Ipilimumab | HCA330 | E | 0.1 mg |
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Human anti Ipilimumab (Drug/Target Complex) | HCA331 | E | 0.1 mg |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
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