TNF Alpha Antibody

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Bovine TNF alpha ELISA

Recombinant Bovine TNF alpha (PBP035) detected using Rabbit anti Bovine TNF alpha (AHP2383) as the capture reagent and Rabbit anti Bovine TNF alpha:Biotin (AHP2383B) as the detection reagent followed by Streptavidin:HRP

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  • Rabbit anti Bovine TNF Alpha
  • Rabbit anti Bovine TNF Alpha:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2383E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2383
    AHP2383BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP2383B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Bovine TNF alpha antibody recognizes bovine tumour necrosis factor alpha (TNF alpha).

      TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells. TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine TNF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant bovine tumour necrosis factor alpha (TNF-α)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      Total protein concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
    • GO Terms
      extracellular space
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1.0 ug/ml10 ug/ml
      Western Blotting0.1 ug/ml1.0 ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.05 ug/ml0.5 ug/ml
      Western Blotting0.1 ug/ml1.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used as a capture antibody in a sandwich ELISA together with AHP2383B as the detection reagent. Recombinant bovine TNF-alpha (PBP035) may be used as a standard.
    • ELISA
      This product may be used as a detection antibody in a sandwich ELISA together with AHP2383 as the capture reagent. Recombinant bovine TNF-alpha (PBP035) may be used as a standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional TNF Alpha Antibody Formats

    Formats Applications Sizes available
    TNF Alpha Antibody : Purified E, WB 0.1 mg
    TNF Alpha Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Bovine TNF Alpha:BiotinAHP2383B50 µgE, WB
          AHP2383B
          Recombinant Bovine TNF AlphaPBP0355 µgE
          PBP035
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Bovine TNF AlphaAHP23830.1 mgE, WB
          AHP2383
          Recombinant Bovine TNF AlphaPBP0355 µgE
          PBP035

          Recommended Positive Controls

            Histology Controls

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