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TNF Alpha

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Bovine TNF alpha ELISA

Recombinant bovine TNF alpha (PBP035) detected using Rabbit anti Bovine TNF alpha (AHP2383) as the capture reagent and Rabbit anti Bovine TNF alpha:Biotin (AHP2383B) as the detection reagent followed by Streptavidin:HRP

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  • Recombinant Bovine TNF Alpha
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP035Edatasheet pdfdatasheet pdf5 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Bovine tumor necrosis factor alpha (TNF alpha), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2), corresponds to the 77 - 234 aa sequence of native bovine TNF alpha. This recombinant protein is produced in yeast cells and is naturally folded and post translationally modified.

      Bovine TNF alpha is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes and is an important mediator of the bovine inflammatory response.

      This product may be used as a standard in immunoassays.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      17.4 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at -20oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      extracellular space
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2383 as a coating antibody and AHP2383B as a detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional TNF Alpha Formats

    Formats Applications Sizes available
    TNF Alpha : Rec. Protein E 5 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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