MBP Antibody | 14

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MBP Antibody | 14 gallery image 1

Published customer image:
Rat anti Bovine myelin basic protein antibody, clone 14 (MCA408S) used for the localization of MBP in mouse brain by immunofluorescence on formalin fixed, frozen sections
Image caption:
MBP1-EGFP expression in neuron soma and neurites
Brains of Tg-MBP1-EGFP mice showed globally strong endogenous green fluorescence (E) and MBP1 immunostaining (F) compared to wild-type mice (A, B). MBP1-EGFP expression was detected in the cell bodies and neurites (as designated by the arrows) in the cortex (G) and CA1 of hippocampus (H) but not in the wild-type mice (C, D). MBP1-EGFP co-localized with neuronal NeuN immunolabeling in cortex (I–L) and CA1 region (M–P). Endogenous green fluorescence (I, M), MBP1 immunolabeling (J, N) and NeuN immunolabeling (K, O) were merged in (L) and (P). Scale bars = 1 mm in A, B, E, F and 50 μm in the remaining panels.

From: Ou-Yang MH, Xu F, Liao MC, Davis J, Robinson JK, Van Nostrand WE.
N-terminal region of myelin basic protein reduces fibrillar amyloid-β deposition in Tg-5xFAD mice.
Neurobiol Aging. 2015 Feb;36(2):801-11.

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  • Rat anti MBP (aa36-50)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    14
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA408SC*, E, IFdatasheet pdfdatasheet pdf2 ml
    MCA408S
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti MBP antibody, clone 14 recognizes myelin basic protein (MBP), a component of myelin that is beleived to play a role in the myelination of nerves in the central nervous system.

      Clone 14 has also been reported to work in Western blotting.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      ChickenExpected from Sequence
      HorseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Bovine myelin basic protein
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised outbred rats were fused with cells of the mouse NS0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      plasma membrane
      protein binding
      myelin sheath
      structural constituent of myelin sheath
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MBP Antibody Formats

    Formats Clone Applications Sizes available
    MBP Antibody : S/N 14 C*, E, IF 2 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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