Bevacizumab Antibody | AbD17976

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Human Anti-Bevacizumab Antibody specificity ELISA

A microtitre plate was coated overnight using various antigens (A: Human Serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Ustekinumab, E: Infliximab, F: Adalimumab, G: Alemtuzumab and H: Bevacizumab) as coating components followed by Human Anti-Bevacizumab Antibody (HCA182) and Mouse anti Strep-tag:HRP (MCA2489P) as the detection reagent.

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Human Anti-Bevacizumab Antibody bridging ELISA

Human Anti-Bevacizumab (Avastin) (clone AbD17976, HCA182) was used as the coating component followed by human serum spiked with increasing concentrations of bevacizumab. The detection reagent was HRP conjugated Human Anti-Bevacizumab Antibody (clone AbD16748, HCA184).

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Human Anti-Bevacizumab Antibody inhibition ELISA

A microtiter plate was coated over night with human VEGF at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of bevacizumab (0.3 μg/ml) plus increasing concentrations of Human Anti-Bevacizumab Antibody (clone AbD17976, HCA182) in the monovalent Fab format was added. Free bevacizumab, still capable of binding to the human VEGF coated plate, was detected using a HRP-conjugated Mouse Anti-Human IgG (Fc) specific Antibody (MCA647P) and QuantaBlu fluorogenic peroxidase substrate. Data is shown as the mean of three measurements.

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  • Human anti Bevacizumab
  • Human anti Bevacizumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-bevacizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of bevacizumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA with HCA184/HCA184P for detection.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD17976
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA182Edatasheet pdfdatasheet pdf0.1 mg
      HCA182
      HCA182GEdatasheet pdfdatasheet pdf1 mg
      HCA182G
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-Bevacizumab Antibody, clone AbD17976 is a paratope specific, recombinant, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug bevacizumab and inhibits the binding of the drug to its target, vascular endothelial growth factor A (VEGF-A). The antibody can be used to measure the levels of free bevacizumab and biosimilar products using bioanalytical assays.

        This antibody is available in two pack sizes, which have different concentrations, 0.1 mg at concentration 0.5 mg/ml, and 1 mg at 1.0 mg/ml.

        A pair of anti-bevacizumab antibodies can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with Anti-Bevacizumab Antibody in full immunoglobulin format, clone AbD16748_hIgG1 (HCA184P) as the detection antibody.

        Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.

        View a summary of all anti-bevacizumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        StrepTactin affinity chromatography
        StrepTactin affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Bevacizumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
      • Approx. Protein Concentrations
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

        Avastin is a trademark of Genentech, Inc.
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

        Avastin is a trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA184P as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA184P as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Bevacizumab Antibody Formats

      Formats Clone Applications Sizes available
      Bevacizumab Antibody : Purified AbD17976 E 0.1 mg | 1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
        MCA1360A
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        MCA1360B
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        MCA1360P
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        MCA2489P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
        MCA1360A
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        MCA1360B
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        MCA1360P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti BevacizumabHCA1840.1 mgE
            HCA184
            Human anti BevacizumabHCA1850.1 mgE
            HCA185
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            Human anti BevacizumabHCA1840.1 mgE
            HCA184
            Human anti BevacizumabHCA1850.1 mgE
            HCA185

            Recommended Positive Controls

              Histology Controls

                References

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