Bevacizumab Antibody | AbD17976

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Human anti bevacizumab specificity ELISA

A microtitre plate was coated overnight using various antigens (A: Human Serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Ustekinumab, E: Infliximab, F: Adalimumab, G: Alemtuzumab and H: Bevacizumab) as coating components followed by Human anti Avastin® (HCA182) and Mouse anti Strep-tag:HRP (MCA2489P) as the detection reagent

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Human anti bevacizumab bridging ELISA

Human anti bevacizumab (Avastin®) (clone AbD17976, HCA182) was used as the coating component followed by human serum spiked with increasing concentrations of bevacizumab. The detection reagent was HRP conjugated Human anti bevacizumab (clone AbD16748, HCA184)

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Human anti bevacizumab inhibition ELISA

A microtiter plate was coated over night with human VEGF at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of bevacizumab (0.3 μg/ml) plus increasing concentrations of human anti bevacizumab (clone AbD17976, HCA182) in the monovalent Fab format was added. Free bevacizumab, still capable of binding to the human VEGF coated plate, was detected using a HRP-conjugated mouse anti human IgG (Fc) specific antibody (MCA647P) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements

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  • Human anti Bevacizumab
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    Anti-bevacizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of bevacizumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA with HCA184/HCA184P for detection.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA182Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Bevacizumab, also known as Avastin, is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to the vascular endothelial growth factor A (VEGF-A). VEGF-A stimulates new blood vessel formation, also in certain diseases, especially in cancer. Avastin is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian. Clinical trials are underway for many other indications.

        Clone AbD17976 is an anti-idiotypic antibody that specifically recognizes the Avastin humanised monoclonal antibody. This antibody can be used to measure the Avastin levels in serum from patients.

        Clone AbD17976 is an affinity-matured antibody. The monovalent intrinsic affinity of Clone AbD17976 was measured as Kd = 0.4 nM by real time, label free molecular interaction analysis using an Attana A200 instrument on immobilized Avastin.

        Additional Bevacizumab (Avastin) antibodies are also available from Bio-Rad.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        StrepTactin affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.

        Avastin® is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA184P as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Bevacizumab Antibody Formats

      Formats Clone Applications Sizes available
      Bevacizumab Antibody : Purified AbD17976 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti V5-Tag:Alk. Phos.MCA1360A0.1 mgE, WB
        Mouse anti V5-Tag:BiotinMCA1360B0.1 mgC, E, WB
        Mouse anti V5-Tag:HRPMCA1360P0.1 mgE, WB
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK005P1 Conjugation For 20mg AntibodyCJ
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LYNX Rapid HRP Antibody Conjugation KitLNK003P1 Conjugation For 4mg AntibodyCJ
            LYNX Rapid HRP Antibody Conjugation KitLNK006P3 Conjugations For 40µg AntibodyCJ
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LYNX Rapid HRP Antibody Conjugation KitLNK004P5 Conjugations For 4mg AntibodyCJ
            Human anti BevacizumabHCA1840.1 mgE
            Human anti BevacizumabHCA1850.1 mgE

            Recommended Positive Controls

              Histology Controls


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