Alemtuzumab Antibody | AbD19189

Alemtuzumab Antibody | AbD19189 gallery image 1

HCA198 specificity ELISA using various antigens as coating reagents. Human anti Alemtuzumab (HCA198) followed by an HRP conjugated anti His tag were used as detection reagents

Alemtuzumab Antibody | AbD19189 gallery image 2

HCA198 PK ELISA. Human anti Alemtuzumab (HCA198) was used as the coating reagent with Alemtuzumab spiked in human serum as the antigen and Human anti Alemtuzumab:HRP (HCA175P) as the detection reagent

Alemtuzumab Antibody | AbD19189 gallery image 3

Binding of FITC conjugated Alemtuzumab to human lymphocytes (blue unshaded histogram) and subsequent inhibition of binding (red shaded histogram) in the presence of a 5 fold molar excess of HCA198 (A) or the corresponding negative control (B)

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  • Human anti Alemtuzumab
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    Anti-alemtuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development to measure levels of alemtuzumab or biosimilars. It is recommended as capture antibody in PK bridging ELISA with HCA175/HCA175P.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA198Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti Alemtuzumab antibody, clone AbD19189 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

        Human anti Alemtuzumab antibody, clone AbD19189 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.

        This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD19189_hIgG1 or clone AbD16942.

        Clone AbD19189 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

        View a summary of all anti-alemtuzumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography.
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        His-tag is a registered trademark of EMD Biosciences.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or as a capture antibody in a bridging ELISA together with HCA175P or HCA199 as the detection reagent when conjugated to HRP.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Alemtuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Alemtuzumab Antibody : Purified AbD19189 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        Rabbit anti DYKDDDDK TagAHP1074GA0.1 mgE, WB
        Mouse anti Histidine TagMCA1396GA0.1 mgE, IP, WB
        Rat anti DYKDDDDK TagMCA4764GA0.1 mgE, F, IF, IP, P, WB

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Alemtuzumab:HRPHCA175P0.1 mgE
            Human anti AlemtuzumabHCA1740.1 mgE
            Human anti AlemtuzumabHCA1750.1 mgE
            Human anti AlemtuzumabHCA1990.1 mgE
            Hispec Assay DiluentBUF049A50 mlIY

            Recommended Positive Controls

              Histology Controls

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