Trastuzumab Antibody | AbD18018

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Herceptin® bridging ELISA using Human anti Herceptin® (HCA168) as the coating antibody; Herceptin® as the antigen; and Human anti Herceptin® (HCA166) conjugated to HRP as the detection antibody

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Herceptin® bridging ELISA using Human anti Herceptin® (HCA166) as the coating antibody; Herceptin® as the antigen and Human anti Herceptin® (HCA168) conjugated to HRP as the detection antibody

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Herceptin® ELISA using Human anti Herceptin® (HCA168) as the coating antibody; Herceptin® as the antigen and Mouse anti Human IgG (Fc):HRP (MCA647P) as the detection antibody

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HCA168 specificity ELISA using various antigens (A: BSA, B: GST, C: Alemtuzumab, D: IgG1/kappa from human myeloma plasma, E: Herceptin®) as coating components; Human anti Herceptin® (HCA168) as the primary antibody and an anti HIS-tag conjugated to HRP as the detection antibody

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Inhibition of Herceptin®-HER2 interaction with Human anti Herceptin® (HCA168). Recombinant HER2 protein was used as the coating antigen followed by a preincubated mixture of the anti-idiotypic antibody HCA168 titrated into a constant amount of Herceptin®. Free Herceptin® still capable of binding to the plate was detected using HRP conjugated anti-human Fab (STAR126P)

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  • Human anti Trastuzumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format, ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated for direct ELISA and as capture or detection antibody in PK bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA168Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti trastuzumab, clone AbD18018, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.

        This antibody is available in two pack sizes, which have different concentrations, 0.1 mg at concentration 0.5 mg/ml, and 1 mg at 1 mg/ml.

        Clone AbD18018 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture or detection antibody paired with antibody clone AbD16712 (HCA166) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.

        This clone is also available in full immunoglobulin format as IgG1 isotype (HCA177) or IgG4 isotype (HCA270) in order to offer assay developers increased flexibility in assay design.

        Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        View a summary of all anti-trastuzumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        HCA168 is an ultra-high affinity idiotype antibody that specifically recognises the Herceptin humanised monoclonal antibody. The monovalent intrinsic affinity of HCA168 was measured as 0.02nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. . This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin; is a registered trademark of Genentech, Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro research testing services. Any re-sale or any other form of commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA together with HCA166 as the detection reagent, when conjugated to HRP.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified AbD18018 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-FH Negative ControlHCA0450.1 mgC, E, P, WB

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
            Human anti TrastuzumabHCA1660.1 mgE
            Human anti TrastuzumabHCA1670.1 mgE
            Human anti TrastuzumabHCA1690.1 mgE
            Human anti TrastuzumabHCA1760.1 mgE
            Human anti TrastuzumabHCA1770.1 mgE
            Hispec Assay DiluentBUF049A50 mlIY

            Recommended Positive Controls

              Histology Controls

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