Trastuzumab Antibody | AbD18018

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Trastuzumab Antibody | AbD18018 gallery image 1

Human Anti-Trastuzumab Antibody capture ELISA

A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody, clone AbD18018 (HCA168) at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, increasing concentrations of trastuzumab in PBST were added. Detection was performed using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data is presented as the mean of three measurements.

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Human Anti-Trastuzumab Antibody bridging ELISA for pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Trastuzumab Antibody, clone AbD16712 (HCA166) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using HRP conjugated Human Anti-Trastuzumab Antibody, clone AbD18018 (HCA168) at a concentration of 4 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation was performed using a LYNX Rapid HRP Conjugation Kit.

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Human Anti-Trastuzumab Antibody specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% milk powder, Human Anti-Trastuzumab Antibody clone AbD18018 (HCA168) was added at a concentration of 2 µg/ml. Detection was performed using Mouse Anti-Penta Histidine Tag:HRP (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.

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Human Anti-Trastuzumab Antibody inhibition ELISA

A microtiter plate was coated overnight with recombinant human HER2 protein at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, trastuzumab was added, spiked with increasing concentrations of Human Anti-Trastuzumab Antibody, clone AbD18018 (HCA168). Free trastuzumab still capable of binding to the HER2 coated plate was detected using Goat Anti-Human IgG F(ab')2:HRP (STAR126P) in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.

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  • Human anti Trastuzumab
  • Human anti Trastuzumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-trastuzumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format, ideal for bioanalytical assays to measure levels of trastuzumab or biosimilars. It has been validated for direct ELISA and as capture or detection antibody in PK bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18018
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA168Edatasheet pdfdatasheet pdf0.1 mg
      HCA168
      HCA168GEdatasheet pdfdatasheet pdf1 mg
      HCA168G
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-Trastuzumab Antibody, clone AbD18018, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug trastuzumab and inhibits it binding to its target. The antibody can be used to measure the levels of trastuzumab and biosimilar products in bioanalytical assays.

        This antibody is available in two pack sizes, which have different concentrations, 0.1 mg at concentration 0.5 mg/ml, and 1 mg at 1.0 mg/ml.

        Clone AbD18018 is a fully human recombinant monoclonal antibody in monovalent Fab format and is suitable as a capture or detection antibody paired with antibody clone AbD16712 (HCA166) in a pharmacokinetic (PK) bridging ELISA, to measure free drug in preclinical research and clinical trials using patient sera.

        This clone is also available in full immunoglobulin format as IgG1 isotype (HCA177) or IgG4 isotype (HCA270) in order to offer assay developers increased flexibility in assay design.

        Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 over-expressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

        View a summary of all anti-trastuzumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Trastuzumab
      • Purity
      • Affinity
        HCA168 is an ultra-high affinity idiotype antibody that specifically recognises the Herceptin humanised monoclonal antibody. The monovalent intrinsic affinity of HCA168 was measured as 0.02nM by real time, label-free molecular interaction analysis using an Attana A200 instrument on immobilized Herceptin.
      • Approx. Protein Concentrations
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin is a registered trademark of Genentech, Inc.
        His-tag is a registered trademark of EMD Biosciences.
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Herceptin is a registered trademark of Genentech, Inc.
        His-tag is a registered trademark of EMD Biosciences.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro research testing services. Any re-sale or any other form of commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA together with HCA166 as the detection reagent, when conjugated to HRP.
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in a direct ELISA or as a capture reagent in a sandwich ELISA together with HCA166 as the detection reagent, when conjugated to HRP.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Trastuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Trastuzumab Antibody : Purified AbD18018 E 0.1 mg | 1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        MCA1396A
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        AHP1656B
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        MCA1396B
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        AHP1074P
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        MCA4764P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        MCA1396A
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        AHP1656B
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        MCA1396B
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        AHP1074P
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        MCA4764P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-FH Negative ControlHCA0450.1 mgC, E, P, WB
          HCA045
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          HuCAL Fab-FH Negative ControlHCA0450.1 mgC, E, P, WB
          HCA045

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
            MCA647P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1670.1 mgE
            HCA167
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Human anti TrastuzumabHCA1760.1 mgE
            HCA176
            Human anti TrastuzumabHCA1770.1 mgE
            HCA177
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
            MCA647P
            Human anti TrastuzumabHCA1660.1 mgE
            HCA166
            Human anti TrastuzumabHCA1670.1 mgE
            HCA167
            Human anti TrastuzumabHCA1690.1 mgE
            HCA169
            Human anti TrastuzumabHCA1760.1 mgE
            HCA176
            Human anti TrastuzumabHCA1770.1 mgE
            HCA177
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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