Trastuzumab antibody | AbD16712

Human anti Trastuzumab

Product Type
Monoclonal Antibody
Clone
AbD16712
Isotype
HuCAL Fab monovalent
Specificity
Trastuzumab

Product Code Applications Pack Size List Price Your Price Qty
HCA166
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E 0.1 mg loader
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Human anti Trastuzumab antibody, clone AbD16712 a monoclonal antibody recognizes Trastuzumab, also known as Herceptin, is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Herceptin binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

Human anti Trastuzumab (AbD16712) is an anti-idiotypic antibody that specifically recognizes the Herceptin humanised monoclonal antibody and can be used to measure the Heceptin levels in serum from patients.

This clone has been demonstrated to inhibit the Herceptin - HER2 interaction. Removal of the preservative is recommended prior to use in functional assays.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. Supplied as a liquid.
Preparation
StrepTactin affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Herceptin
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.2 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Herceptin® is a registered trademark of Genentech, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection antibody, or as capture antibody in a sandwich ELISA together with HCA168, when conjugated to HRP, as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti V5-Tag:Alk. Phos. MCA1360A E WB 0.1 mg loader
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Description Mouse anti V5-Tag:Alk. Phos.
Mouse anti V5-Tag:Biotin MCA1360B C E WB 0.1 mg loader
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Description Mouse anti V5-Tag:Biotin
Mouse anti V5-Tag:HRP MCA1360P E WB 0.1 mg loader
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Description Mouse anti V5-Tag:HRP
Mouse anti Strep-Tag Immo MCA2488 E 0.1 mg loader
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Description Mouse anti Strep-Tag Immo
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader
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Description Mouse anti Strep-Tag Classic:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Trastuzumab HCA167 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA168 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA169 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA176 E 0.1 mg loader
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Description Human anti Trastuzumab
Human anti Trastuzumab HCA177 E 0.1 mg loader
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Description Human anti Trastuzumab
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP

Synonyms
HERCEPTIN®
RRID
AB_11152937
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"