IL-6 Antibody | 4B6

IL-6 Antibody | 4B6 gallery image 1

Published customer image:
Mouse anti Ovine Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody in a sandwich ELISA for the evaluation of IL-6 expression in response to LPS treatment using Rabbit anti Ovine IL-6 antibody (AHP424) as a detection reagent.
Image caption:
Fetal inflammatory response to lipopolysaccharide.
Blue, control group (n = 5); red, LPS group (n = 10); Mean ± SD. *, P = 0.001 versus control.

From: Herry CL, Cortes M, Wu H-T, Durosier LD, Cao M, Burns P, et al. (2016)
Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
PLoS ONE 11(4): e0153515.

IL-6 Antibody | 4B6 gallery image 2

Published customer image:
Mouse anti Sheep interleukin 6 antibody, clone 4B6 (MCA1659) used as a capture reagent for the measurement of interleukin 6 in an ELISA with Rabbit anti Sheep polyclonal antibody (AHP424) as a detection reagent using lamb sera samples. Interleukin 1β was detected with Mouse anti Sheep IL-1β antibody (MCA1658) as a capture reagent and Rabbit anti IL-1β polyclonal antibody (AHP423) as a detection reagent. Visualisation was achieved with a Goat anti Rabbit IgG antibody conjugated to HRP in both cases.
Image caption:
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.

From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.

IL-6 Antibody | 4B6 gallery image 3

Published customer image:
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody for the measurement of IL-6 levels in sheep plasma by ELISA with Rabbit anti Sheep IL-6 polyclonal antibody (AHP424) used as a detection reagent.
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p ≤ 0.0001.

From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015)
Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.

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  • Mouse anti Sheep Interleukin-6
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1659E, F*, WBdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Sheep Interleukin-6 antibody, clone 4B6 recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant ovine IL-6.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      immune response
      acute-phase response
      growth factor activity
      extracellular space
      interleukin-6 receptor binding
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml (as a coating antibody)
      Western Blotting
      Flow Cytometry(1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      MCA1659 may also be used in combination with AHP424 in a sandwich ELISA assays for ovine IL-6.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-6 Antibody Formats

    Formats Clone Applications Sizes available
    IL-6 Antibody : Purified 4B6 E, F*, WB 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images


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