IL-6 Antibody

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IL-6 Antibody gallery image 1

Published customer image:
Mouse anti Sheep Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody for the measurement of IL-6 levels in sheep plasma by ELISA with Rabbit anti Sheep IL-6 polyclonal antibody (AHP424) used as a detection reagent.
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p ≤ 0.0001.

From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015)
Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.

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IL-6 Antibody gallery image 2

Published customer image:
Mouse anti Ovine Interleukin-6 antibody, clone 4B6 (MCA1659) used as a capture antibody in a sandwich ELISA for the evaluation of IL-6 expression in response to LPS treatment using Rabbit anti Ovine IL-6 antibody (AHP424) as a detection reagent.
Image caption:
Fetal inflammatory response to lipopolysaccharide.
Blue, control group (n = 5); red, LPS group (n = 10); Mean ± SD. *, P = 0.001 versus control.

From: Herry CL, Cortes M, Wu H-T, Durosier LD, Cao M, Burns P, et al. (2016)
Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
PLoS ONE 11(4): e0153515.

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IL-6 Antibody gallery image 3

Published customer image:
Mouse anti Sheep interleukin 6 antibody, clone 4B6 (MCA1659) used as a capture reagent for the measurement of interleukin 6 in an ELISA with Rabbit anti Sheep polyclonal antibody (AHP424) as a detection reagent using lamb sera samples. Interleukin 1β was detected with Mouse anti Sheep IL-1β antibody (MCA1658) as a capture reagent and Rabbit anti IL-1β polyclonal antibody (AHP423) as a detection reagent. Visualisation was achieved with a Goat anti Rabbit IgG antibody conjugated to HRP in both cases.
Image caption:
Systemic and diaphragm cytokine responses. Plasma IL-1β (A) and IL-6 (B) protein content. Values are mean ± SEM. Diaphragm IL-1β (C) and IL-6 (D) mRNA expression. Values are median (with 10th and 90th centiles). * p<0.05 compared to Sal/Sal; # p<0.05 compared to Sal/LPS.

From: Karisnan K, Bakker AJ, Song Y, Noble PB, Pillow JJ, et al. (2015)
Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS ONE 10(4): e0124390.

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  • Rabbit anti Sheep Interleukin-6
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP424E, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP424
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Ovine interleukin-6 antibody recognizes ovine interleukin-6 (IL-6). No cross-reactivity is seen with ovine IL-1 beta, IL-8, MCP or TNF alpha.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Serum - liquid (diluted)
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ovine IL-6 were raised by repeated immunisation of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant IL-6.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      interleukin-6 receptor binding
      growth factor activity
      cytokine activity
      immune response
      acute-phase response
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/500
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      The antibody may be used in a sandwich ELISA as a detection reagent for ovine IL-6, in combination with MCA1659 as a capture antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-6 Antibody Formats

    Formats Applications Sizes available
    IL-6 Antibody : Serum E, WB 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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