Antibody conjugation in a range of fluorophores
Use ReadiLink Antibody Conjugation Kits to quickly conjugate microscale volumes of antibody (50-100 µg) in two easy steps with the unique chemistry of ReadiLink Dyes. Fluorophores are available that are excited by UV all the way to infrared with a range of emission spectra to easily fit into your multicolor flow cytometry experiments, giving you greater choice.
Figure 1. Efficient labeling of antibodies in two easy steps
The unique chemistry of ReadiLink Dyes allows you to quickly label your antibody in just two easy steps, shown in Figure 1.
Each ReadiLink Dye Kit includes succinimidyl esters that selectively react with primary amines to form a stable carboxamide bond. This bond ensures no dissociation between the fluorophore and antibody which may lead to unbound fluorophore in you experiments.
Saving you time: As no purification column required
Minimal hands on time: Easy protocol doesn’t involve complicated chemistry, allowing you to perform other experiments
Ready-to-use: No fluorophore quenching required
Versatile: Available in 13 unique fluorophores with a wide range of excitation wavelengths – giving you greater flexibility in your multicolor flow cytometry and cell sorting experiments as well as other applications such as western blot and ELISA
The table below shows the range of available ReadiLink Dyes, maximal excitation and emission properties, and their fluorophore equivalents to enable you to choose the right fluorophore for your flow cytometry experiment.
In addition to conjugation kits we have flow cytometry validated antibodies, reagents and resources to help you optimize your flow cytometry.
ReadiLink™ is a trademark of AAT Bioquest, Inc.
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