Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Please amend your browser settings to enable third-party cookies and access this website’s full functionality.
Readilink Antibody Conjugation Kits
Readilink Kits offer a simple antibody solution for labeling microscale amounts (50-100 µg) of antibody for flow cytometry and cell sorting applications in just over an hour. For ease of use each kit contains 2 vials of dye, each vial is for 50 µg of antibody, or other protein greater than 10 kDa in size.
The Readilink Antibody Labeling Kits quickly conjugate in two easy steps, label and quench. After the conjugation reaction and addition of quencher buffer, any unbound dye will bind to the quencher and become non-fluorescent.
There are 12 fluorophores available, most of which are ideal for flow cytometry. A wide spectrum of wavelengths ranging from violet to infrared is offered for greater flexibility in multicolor assay design.
For larger scale reactions and conjugations with alternative labels such as enzymes and biotin, please see our range of LYNX Rapid Conjugation Kits®.
The reactive dyes in Readilink Antibody Conjugation Kits selectively bind the primary amines of proteins to form a stable carboxamine bond. The kits have been developed to label purified monoclonal or polyclonal antibodies, however other proteins greater than 10 kDa in size can also be labeled. Each kit can label up to 100 µg of antibody (2 x 50 µg reactions).
The antibody should be purified, as all amine containing proteins will be labeled. Antibodies in formats such as ascites, tissue culture supernatant or serum are unsuitable, as well as those containing BSA or gelatin, as all proteins will be conjugated to the fluorophore. Conjugation efficiency is significantly reduced if antibody concentration is less than 1 mg/ml. For optimal labeling efficiency, a concentration of 1-2 mg/ml is recommended.
Antibodies for conjugation should preferably be prepared in phosphate buffered saline (PBS) pH 7.2 -7.4, but HEPES, MES, MOPS or other amine-free buffers are also suitable. Amine containing buffers such as Tris buffer and glycine buffer are unsuitable. If the antibody is in these buffers it will require dialysis against PBS to remove free amines or ammonium salts.
Buffer containing less than 0.1% sodium azide or 10% methanol should not interfere with labeling.
It takes just 1-2 min of your time to conjugate an antibody with the Readilink Conjugation Kits.
The antibody conjugation is complete in 70 min with only two hands-on steps required.
Antibodies conjugated using the Readilink Kit require no post-conjugation clean up processes (column purification, dialysis/desalting). After conjugation and addition of the quencher buffer, any unbound dye will bind to the quencher and become non-fluorescent. The antibody is ready to use immediately.
The conjugated antibody should be stored at a concentration of at least 0.5 mg/ml. Addition of antimicrobial agents, preservatives, or stabilizers (e.g sodium azide, BSA, glycerol, etc) is strongly recommended for storage at 4°C. For long term storage, the protein conjugates can be lyophilized or divided into single-use aliquots and stored at ≤ –20 °C.
If you have any other questions, contact our technical support team.