Murine Vascular Endothelial Growth Factor (VEGF) is a homodimeric protein, where each subunit is 165 amino acids in length.
Murine VEGF is expressed by various vascularised tissues and is reported to stimulate endothelial cell growth and angiogenesis.
Purified Recombinant Protein - lyophilised
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Recombinant protein expressed in E. coli.
>98% by SDS page and HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
39kD (homodimer of 165 amino acid sequence)
Reagents In The Kit
Preparing The Antibody
0.1% Trifluoroacetic acid.
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using apprpriate negative/positive controls.
PMP67 may be used in ELISA applications with either AAM51 or AAM51B.