Interleukin-1 beta (IL-1 beta), is a 17.5 kDa proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumour cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.
Purified recombinant protein - lyophilised
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant murine IL-1 beta, expressed in E. coli
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of > 5x108 units/mg.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
17.5kD (153 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
PMP71 may be used as an ELISA standard for our AAM13G and AAM13B antibodies.
Histology Positive Control Tissue
PMP71 may be used as a postive control for Western blotting applications with our AAM13G and AAM13B antibodies.