GAPDH antibody
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WB C IF ResetRabbit anti GAPDH (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- GAPDH
- Region
- (N-TERMINAL)
Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair. GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human GADPH (N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- AHP1628T: Phosphate buffered saline.
- AHP1628: Phosphate buffered saline
- Preservative Stabilisers
- AHP1628T: <0.1% Sodium Azide (NaN3)
- AHP1628: 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunocytochemistry | 1/25 | 1/100 | |
Immunohistology - Paraffin 1 | 10ug/ml | ||
Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human liver tissue
- Western Blotting
- AHP1628 detects a band of approximately 40kDa in HeLa cell lysates.
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References for GAPDH antibody
-
Mohan, H. et al. (2014) Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice.
PLoS One. 9 (12): e115102. -
Rogalska, A. et al. (2014) Epothilone B induces extrinsic pathway of apoptosis in human SKOV-3 ovarian cancer cells.
Toxicol In Vitro. 28: 675-83. -
Paré, B. et al. (2015) Early detection of structural abnormalities and cytoplasmic accumulation of TDP-43 in tissue-engineered skins derived from ALS patients.
Acta Neuropathol Commun. 3 (1): 5. -
Antonucci, J.M. et al. (2016) SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
Nat Med. 22 (10): 1072-1074. -
Nevzorova, Y.A. et al. (2017) Anti-tumorigenic and anti-angiogenic effects of natural conifer Abies sibirica terpenoids in vivo and in vitro.
Biomed Pharmacother. 89: 386-95. -
Pham, D. et al. (2022) Inhibition of TGFb-induced Fibroblast to Myofibroblast Transition by Conditioned Medium from Human Rhinovirus-infected Airway Epithelial Cells is Mediated by Prostaglandin E2
Res Sq 21 Jan. [Epub ahead of print]. -
Phillips, S. et al. (2022) Protocol for the generation of HIV-1 genomic RNA with altered levels of N6-methyladenosine
STAR Protocols. 3 (3): 101616. -
Espada, C.E. et al. (2023) SAMHD1 impairs type I interferon induction through the MAVS, IKKε, and IRF7 signaling axis during viral infection.
J Biol Chem. 299 (7): 104925.
View The Latest Product References
-
Kopf, A. et al. (2020) Microtubules control cellular shape and coherence in amoeboid migrating cells.
J Cell Biol. 219 (6): e201907154.
- Synonyms
- GLYCERALDEHYDE-3-PHOSPHATE Dehydrogenase
- RRID
- AB_1604986
- UniProt
- P04406
- Entrez Gene
- GAPDH
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- View More GO Terms
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
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