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GAPDH antibody

Rabbit anti GAPDH (N-Terminal)

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IC P * WB 50 µg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IC P * WB 0.1 mg loader
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Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.

GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human GADPH (N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
AHP1628T: Phosphate buffered saline.
AHP1628: Phosphate buffered saline
Preservative Stabilisers
AHP1628T: <0.1% Sodium Azide (NaN3)
AHP1628: 0.02% Sodium Azide (NaN3)
A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/25 1/100
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human liver tissue
Western Blotting
AHP1628 detects a band of approximately 40kDa in HeLa cell lysates.

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References for GAPDH antibody

  1. Mohan, H. et al. (2014) Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice.
    PLoS One. 9 (12): e115102.
  2. Rogalska, A. et al. (2014) Epothilone B induces extrinsic pathway of apoptosis in human SKOV-3 ovarian cancer cells.
    Toxicol In Vitro. 28: 675-83.
  3. Paré, B. et al. (2015) Early detection of structural abnormalities and cytoplasmic accumulation of TDP-43 in tissue-engineered skins derived from ALS patients.
    Acta Neuropathol Commun. 3 (1): 5.
  4. Antonucci, J.M. et al. (2016) SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
    Nat Med. 22 (10): 1072-1074.
  5. Nevzorova, Y.A. et al. (2017) Anti-tumorigenic and anti-angiogenic effects of natural conifer Abies sibirica terpenoids in vivo and in vitro.
    Biomed Pharmacother. 89: 386-95.
  6. Pham, D. et al. (2022) Inhibition of TGFb-induced Fibroblast to Myofibroblast Transition by Conditioned Medium from Human Rhinovirus-infected Airway Epithelial Cells is Mediated by Prostaglandin E2
    Res Sq 21 Jan. [Epub ahead of print].
  7. Phillips, S. et al. (2022) Protocol for the generation of HIV-1 genomic RNA with altered levels of N6-methyladenosine
    STAR Protocols. 3 (3): 101616.
  8. Espada, C.E. et al. (2023) SAMHD1 impairs type I interferon induction through the MAVS, IKKε, and IRF7 signaling axis during viral infection.
    J Biol Chem. 299 (7): 104925.
  9. View The Latest Product References
  10. Kopf, A. et al. (2020) Microtubules control cellular shape and coherence in amoeboid migrating cells.
    J Cell Biol. 219 (6): e201907154.

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis


153170 157440 158031 161115 167491


155201 157977 158891 166918

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