Light Chain Antibody | SB62a

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Light Chain Antibody | SB62a gallery image 1

HeLa (lane 2 and 5) and HepG2 (lane 3 and 6) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 4. Rabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP (MCA6003P) was used as the secondary antibody at dilutions of 1/5000 (lanes 2 and 3) and 1/10000 (lanes 5 and 6). Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure

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  • Mouse anti Rabbit Light Chain:HRP
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  • Product Type
    Monoclonal Antibody
  • Clone
    SB62a
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6003PE, WBdatasheet pdfdatasheet pdf0.5 ml
    MCA6003P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rabbit light chain antibody, clone SB62a only binds to the light chain of rabbit IgG and does not bind to the heavy chain. As such it can be used as a secondary antibody in western blotting, EIA and immunoprecipitation. This antibody is not recommended for the direct detection of reduced or denatured rabbit chains on western blots.
    • Intended Use
    • Target Species
      Rabbit
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG from tissue culture supernatant
    • Preservative Stabilisers
      50% Glycerol
    • Immunogen
      Rabbit light chain
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
      Western Blotting1/10001/10000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Light Chain Antibody Formats

    Formats Clone Applications Sizes available
    Light Chain Antibody : HRP SB62a E, WB 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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