BrdU Antibody | Bu20a

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BrdU Antibody | Bu20a gallery image 1

Published customer image:
Mouse anti Bromodeoxyuridine antibody, clone Bu20a used to label DNA synthesis in cultured mouse astocytes by immunofluorescence.
Image caption:
Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 μm. (b) After 24 h, there was a significant 77 ± 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 ± 4%, while KB-R7943 decreased proliferation by 46 ± 9%. *p<0.05; n.s., not significant.

From: Pappalardo LW, Samad OA, Black JA, Waxman SG.
Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 2014 Jul;62(7):1162-75.

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BrdU Antibody | Bu20a gallery image 2

A. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, (MCA2483) at a 1/100 dilution for 1 hour at room temperature. Goat anti Mouse IgG (H/L) DyLight® 649 conjugated (STAR117D649GA) was used as the secondary detection reagent at a 1/50 dilution. PUREBLU™ Hoechst 33342 (1351304) was used to stain total DNA. B. BrdU-labeled human lymphoma cells were stained with Mouse anti BrdU antibody, clone BU20a, (MCA2483) at a 1/100 dilution overnight at 4°C. Goat anti Mouse IgG (H/L) FITC conjugated antibody (STAR117F) was used at a 1/50 dilution. READIDROP™ Propidium Iodide (1351101) was used to stain total DNA.

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BrdU Antibody | Bu20a gallery image 3

Published customer image:
Mouse anti Bromodeoxyuridine antibody, clone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.
(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200μm.

From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.

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BrdU Antibody | Bu20a gallery image 4

Published customer image:
Mouse anti Bromodeoxyuridine antibody, vlone Bu20a used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B-) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20μm.

From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.

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BrdU Antibody | Bu20a gallery image 5

Published customer image:
Mouse anti Bromodeoxyuridine antibody, clone Bu20a used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
BrdU/ Bu20a/ AbD serotec/ MCA2483, Small intestine/ Rat.

From: Furukawa S, Nagaike M, Ozaki K.
Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 2017 Jan;30(1):79-107.

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BrdU Antibody | Bu20a gallery image 6

HeLa cells were treated with 10 µg BrdU for 1 hour (B) or left untreated (A). Cells were stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483) at a dilution of 1/25. As a secondary antibody, goat anti mouse IgG (H/L) DyLight® 549 conjugated antibody (red) (STAR117D549GA) was used at a 1/50 dilution. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628) at a dilution of 1/100. As a secondary antibody, sheep anti rabbit IgG DyLight® 488 conjugated antibody (green) (STAR36D488GA) was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.

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  • Mouse anti BrdU
  • Mouse anti BrdU
  • Mouse anti BrdU
  • Mouse anti BrdU:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
    BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). This BU20a antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.
    • Product Type
      Monoclonal Antibody
    • Clone
      Bu20a
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2483GAC, F *, IC*, Pdatasheet pdfdatasheet pdf0.1 mg
      MCA2483GA
      MCA2483C, F *, IC*, Pdatasheet pdfdatasheet pdf0.2 mg
      MCA2483
      MCA2483TC, F *, IC*, Pdatasheet pdfdatasheet pdf20 µg
      MCA2483T
      MCA2483FAF *datasheet pdfdatasheet pdf50 µg
      MCA2483FA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).

        Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

        BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
      • Intended Use
      • Target Species
        Chemical
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide (NaN3)
        0.09%Sodium Azide (NaN3)
        0.09%Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        Bromodeoxyuridine conjugated to BSA
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch
        18 months from date of despatch.
      • Acknowledgements
      • Regulatory
        For research purposes only

      Additional BrdU Antibody Formats

      Formats Clone Applications Sizes available
      BrdU Antibody : Purified Bu20a C, F *, IC*, P 20 µg | 0.1 mg | 0.2 mg
      BrdU Antibody : FITC Bu20a F * 50 µg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative ControlMCA928100 TestsF
          MCA928
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative ControlMCA928100 TestsF
          MCA928
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative ControlMCA928100 TestsF
          MCA928

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            PUREBLU™ DAPI1351303250 µgF, IF
            1351303
            PUREBLU™ Hoechst 333421351304280 µgF, IF
            1351304
            READIDROP™ Propidium Iodide13511013 X 3mlF
            1351101
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            PUREBLU™ DAPI1351303250 µgF, IF
            1351303
            PUREBLU™ Hoechst 333421351304280 µgF, IF
            1351304
            READIDROP™ Propidium Iodide13511013 X 3mlF
            1351101
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            PUREBLU™ DAPI1351303250 µgF, IF
            1351303
            PUREBLU™ Hoechst 333421351304280 µgF, IF
            1351304
            READIDROP™ Propidium Iodide13511013 X 3mlF
            1351101

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Immunofluorescence
                  Immunohistology - Frozen

                References

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