Caspase-3 antibody
KO Validated
Filter by Application:
WB P ResetRabbit anti Caspase-3
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- Caspase-3
Antibody Quality and Performance Guarantee
| Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3, as the name implies, is a member of the cysteine-aspartic acid protease (caspase) protein family. Caspase-3 is an effector caspase that is activated by both the intrinsic and extrinsic apoptosis pathways. Caspase-3 is cleaved from an inactive 32 kDa zymogen/pro-enzyme into p17 and p12 subunits by the activated initiator caspases 9 and 8 in apoptotic cells. These subunits dimerize to form the active caspase 3 protein that cleaves and activates caspase-6 and 2. Through the activation/cleavage of downstream caspases, caspase-3 plays an important role in mediating apoptosis signaling. |
|
- Target Species
- Human
- Western Blotting
- Rabbit anti caspase-3 antibody detects a band of approximately 32 kDa in Jurkat cell lysate under reducing conditions
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to caspase-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% ProClin 300
50% Glycerol - Immunogen
- Recombinant human caspase-3
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/50 | 1/200 |
| Western Blotting | |
1/500 | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- UniProt
- P42574
- Entrez Gene
- CASP3
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0004197 cysteine-type endopeptidase activity
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0006508 proteolysis
- GO:0008624 induction of apoptosis by extracellular signals
- View More GO Terms
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008635 activation of caspase activity by cytochrome c
- GO:0030264 nuclear fragmentation involved in apoptotic nuclear change
- GO:0034612 response to tumor necrosis factor
- GO:0043066 negative regulation of apoptosis
- GO:0048011 nerve growth factor receptor signaling pathway
AHP2717
141649
154703
158224
163879
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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