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TIM-4 antibody | RMT4-54

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MCA5791GA
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F IF P 0.1 mg loader
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Rat anti Mouse TIM-4 antibody, clone RMT4-54 recognizes mouse Tim-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of Tim-1, involved in T-cell expansion and cytokine production.

Tim-4 is necessary for the phagocytosis of apoptotic cells by peritoneal macrophages (Nishi et al. 2014) and interaction between Tim-1 on Th2 cells and Tim-4 on DCs, enhances Th2 cell function. The TIM-4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.

Rat anti Mouse TIM-4 antibody, clone RMT4-54 inhibits the efficient in vitro phagocytosis of apoptotic cells by resident peritoneal Mac1+ cells), but not inflammatory peritoneal exudate Mac1+ cells (Nakayama et al. 2009).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Tim-4-Ig, consisting of amino acids 1-288 of mouse Tim-4, coupled with the Fc portion of mouse IgG2a.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Lymph node cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

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Source Reference

  1. Nakayama, M. et al. (2009) Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation.
    Blood. 113 (16): 3821-30.

Further Reading

  1. Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
    Immunol Rev. 235 (1): 172-89.

Synonyms
T Cell Immunoglobulin Mucin 4
UniProt
Q6U7R4
Entrez Gene
Timd4
GO Terms
GO:0016021 integral to membrane

MCA5791GA

0811 157508 163606

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