MHC Class I H-2b/D/P/Q/w16 antibody | ER-HR52
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16
- Product Type
- Monoclonal Antibody
- Clone
- ER-HR52
- Isotype
- IgG2a
- Specificity
- MHC Class I H-2b/D/P/Q/w16
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Adherent FI (CBAXBL) bone marrow stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/200 | |
Immunofluorescence | |||
Immunohistology - Frozen |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Rat IgG2a Negative Control |
References for MHC Class I H-2b/D/P/Q/w16 antibody
-
Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
Am J Pathol. 159 (3): 809-15. -
Himoudi, N. et al. (2009) Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Cancer Res. 69 (16): 6598-606. -
Liu, J. et al. (2013) The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
Neurochem Res. 38 (2): 290-9. -
Lv, D. et al. (2014) The similar expression pattern of MHC class I molecules in human and mouse cerebellar cortex.
Neurochem Res. 39 (1): 180-6. -
Liu, J. et al. (2015) Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain.
Neurochem Res. 40 (7): 1487-96. -
Lv, D. et al. (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS One. 10 (8): e0135223. -
Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
Glia. 66 (5): 1034-52.
- RRID
- AB_872030
MCA2398
151412 154514 158077 166387If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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