MHC Class I H-2b/D/P/Q/w16 Antibody | ER-HR52

MHC Class I H-2b/D/P/Q/w16 Antibody | ER-HR52 gallery image 1

Published customer image:
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 (MCA2398) used for the identification of MHC class I expressing cells in mouse brain by immunofluorescence.
Image caption:
Dynamic expression of H-2Kb/Db in mouse hippocampus.
(A) At P15, MHCI protein signals (green) were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 µm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with P4 and was calculated from three independent experiments. (C) Western blot analysis depicting the expression of MHC-I protein during the development stages of hippocampus. (D) Expression of MHC-I protein was quantified as the ratio of band density to that of Tuj1. Data was presented as ratio compared with P4 and was calculated from three independent experiments.

From: Lv D, Shen Y, Peng Y, Liu J, Miao F, Zhang J (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS ONE 10(8): e0135223.

  • MHC Class I H-2b/D/P/Q/w16 Antibody | ER-HR52 thumbnail image 1
  • Rat anti Mouse MHC Class I H-2b/D/P/Q/w16
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2398C, Fdatasheet pdfdatasheet pdf0.25 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.

      Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.

      The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Adherent FI (CBAXBL) bone marrow stromal cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class I H-2b/D/P/Q/w16 Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class I H-2b/D/P/Q/w16 Antibody : Purified ER-HR52 C, F 0.25 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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