M31 Antibody | MAC353

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  • Rat anti M31/HP1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MAC353
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1946C, CH, E, IF, IP, WBdatasheet pdfdatasheet pdf2 ml
    MCA1946
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse M31 antibody, clone MAC353 recognises the M31 molecule in mouse and the homologous Chromobox protein homolog 1 or HP1Hsbeta molecule in man. Rat anti Mouse M31 antibody, clone MAC353 has been reported for use in western blotting and recognises a ~26 kDa protein in murine and human nuclear extracts (Wreggett et al. 1994).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      5% Bovine Serum Albumin
    • Immunogen
      71 amino acid fusion protein corresponding to the C-terminal region of M31
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      negative regulation of transcription, DNA-dependent
      male pronucleus
      chromatin binding
      identical protein binding
      transcription repressor activity
      female pronucleus
      chromatin assembly or disassembly
      centromeric heterochromatin
      chromocenter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Chromatin Immunoprecipitation
      ELISA
      Immunofluorescence
      Immunohistology - FrozenNeat1/50
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional M31 Antibody Formats

    Formats Clone Applications Sizes available
    M31 Antibody : S/N MAC353 C, CH, E, IF, IP, WB 2 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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