IL-1 Beta Antibody

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  • Rabbit anti Mouse Interleukin-1 beta:Biotin
  • Rabbit anti Mouse Interleukin-1 beta
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM13GC, E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM13G
    AAM13BE, WBdatasheet pdfdatasheet pdf50 µg
    AAM13B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse Interleukin-1 beta antibody recognizes mouse Interleukin-1 beta (IL-1 beta), a cytokine involved in the inflammatory response.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to murine IL-1beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by antigen affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant murine IL-1 beta
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50 ug/ml after reconstitution
      IgG concentration 1.0mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of cell division
      interleukin-1 receptor binding
      negative regulation of insulin receptor signaling pathway
      response to lipopolysaccharide
      stored secretory granule
      cytokine activity
      response to ATP
      positive regulation of angiogenesis
      negative regulation of glucose transport
      negative regulation of MAP kinase activity
      positive regulation of interleukin-6 production
      positive regulation of lipid catabolic process
      positive regulation of fever generation
      fever generation
      positive regulation of JNK cascade
      growth factor activity
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      positive regulation of interleukin-6 biosynthetic process
      positive regulation vascular endothelial growth factor production
      negative regulation of adiponectin secretion
      neutrophil chemotaxis
      positive regulation of prostaglandin secretion
      positive regulation of chemokine biosynthetic process
      extracellular space
      positive regulation of gene-specific transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays0.10.15ug/ml
      Immunohistology - Frozen
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      AAM13B may be used in a direct ELISA or as the detection reagent in in a sandwich ELISA with AAM13G as the capture reagent and PMP71 as the standard.
    • ELISA
      AAM13G may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM13B as the detection reagent an PMP71 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AAM13B may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
    • Western Blotting
      AAM13G may be used under either reducing or non-reducing conditions with PMP71 as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IL-1 Beta Antibody Formats

    Formats Applications Sizes available
    IL-1 Beta Antibody : Biotin E, WB 50 µg
    IL-1 Beta Antibody : Purified C, E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Mouse Interleukin-1 betaAAM13G0.1 mgC, E, FN, WB
          AAM13G
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Mouse Interleukin-1 beta:BiotinAAM13B50 µgE, WB
          AAM13B
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Mouse Interleukin-1 betaPMP7110 µgE, FN, WB
            PMP71
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Mouse Interleukin-1 betaPMP7110 µgE, FN, WB
            PMP71

            Histology Controls

              References

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