Delta-Like Protein 1 Antibody | HMD1-5

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Delta-Like Protein 1 Antibody | HMD1-5 gallery image 1

Mouse Delta like protein 1 transfected cells stained with Hamster anti Mouse Delta like protein 1 (MCA5705)

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Delta-Like Protein 1 Antibody | HMD1-5 gallery image 2

Paraffin embedded human spleen stained with Hamster anti Mouse Delta-Like protein 1 (MCA5705) followed by Goat anti Hamster IgG:HRP (STAR79P)

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Delta-Like Protein 1 Antibody | HMD1-5 gallery image 3

Mouse Delta like protein 1 transfected cells stained with Hamster anti Mouse Delta like protein 1:RPE (MCA5705PE)

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  • Delta-Like Protein 1 Antibody | HMD1-5 thumbnail image 1
  • Delta-Like Protein 1 Antibody | HMD1-5 thumbnail image 2
  • Delta-Like Protein 1 Antibody | HMD1-5 thumbnail image 3
  • Hamster anti Mouse Delta-Like Protein 1:RPE
  • Hamster anti Mouse Delta-Like Protein 1
  • Hamster anti Mouse Delta-Like Protein 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HMD1-5
  • Isotype
    IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5705GAC, F, FN*datasheet pdfdatasheet pdf0.1 mg
    MCA5705GA
    MCA5705C, F, FN*datasheet pdfdatasheet pdf0.25 mg
    MCA5705
    MCA5705PEFdatasheet pdfdatasheet pdf100 Tests
    MCA5705PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 specifically recognizes Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.

      The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.

      Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

      DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

      Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 blocks binding of Notch2 to Dll1 (Moriyama et al. 2008)
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Functional Assays(1)
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Functional Assays(1)
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Mouse spleen
    • Histology Positive Control Tissue
      Mouse spleen
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Delta-Like Protein 1 Antibody Formats

    Formats Clone Applications Sizes available
    Delta-Like Protein 1 Antibody : Purified HMD1-5 C, F, FN* 0.1 mg | 0.25 mg
    Delta-Like Protein 1 Antibody : RPE HMD1-5 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Mouse spleen
              Mouse spleen

              References

              Further Reading

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