IgG antibody
Horseradish peroxidase conjugated Goat anti Hamster IgG antibody (STAR79P) used for the assessment of hamster IgG response following immunization with a series of immunogens.
Image caption:
Inducing the humoral immune response in hamsters immunized with different immunogens. Fifty micrograms of the recombinant protein were used. The groups of hamsters were immunized subcutaneously on day 0 and boosted after 2 weeks (day 14). Blood was collected on days 0, 14, and 28 after immunization. The specific IgG responses stimulated by the different immunogens were determined by an ELISA of the hamster serum diluted 1:50. The values presented are the means ± SEM for two independent experiments.
From: Bacelo KL, Hartwig DD, Seixas FK, Schuch R, Moreira Ada S, Amaral M, Collares T, Vendrusculo CT, McBride AJ, Dellagostin OA.
Xanthan gum as an adjuvant in a subunit vaccine preparation against leptospirosis.
Biomed Res Int. 2014;2014:636491.
Product Details
- Target Species
- Hamster
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Antiserum Preparation
- Antisera to hamster IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.01% Thiomersal 1% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 0.8 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.7 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.8 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/5000 | |
| Flow Cytometry | 1/25 | 1/50 | |
| Flow Cytometry | 1/50 | ||
| ELISA | 1/1000 | 1/5000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1 x 106 cells in 100ul.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Samant, M. et al. (2009) Immunization with the DNA-encoding N-terminal domain of proteophosphoglycan of Leishmania donovani generates Th1-type immunoprotective response against experimental visceral leishmaniasis.
J Immunol. 183: 470-9. -
Moore, G.T. et al. (2008) Glycosylation changes in hFUT1 transgenic mice increase TCR signaling and apoptosis resulting in thymocyte maturation arrest.
Mol Immunol. 45: 2401-10. -
Forster, K.M. et al. (2015) DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis.
Mem Inst Oswaldo Cruz. 110 (8): 989-95. -
Verma R et al. (2015) Cross reactive molecules of human lymphatic filaria Brugia malayi inhibit Leishmania donovani infection in hamsters.
Acta Trop. 152: 103-11. -
Wiśniewski, M. et al. (2016) Hamsters vaccinated with Ace-mep-7 DNA vaccine produced protective immunity against Ancylostoma ceylanicum infection.
Exp Parasitol. 163: 1-7. -
Bacelo, K.L. et al. (2014) Xanthan gum as an adjuvant in a subunit vaccine preparation against leptospirosis.
Biomed Res Int. 2014: 636491.
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