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CD44v6 Antibody | 9A4

CD44v6 Antibody | 9A4 gallery image 1

Published customer image:
Rat anti Mouse CD44v6 antibody used for the identification of CD44v6 expressing luminal progenitor cells by immunofluorescence.
Image caption:
Molecular and phenotypic characteristics of luminal progenitors isolated from virgin and early pregnant females. (A) q-PCR analysis of gene expression levels in mammary Lu-neg and Lu-pos cells isolated from early pregnant females (P-8d). Mean ratios of values normalized to Gapdh expression are shown. Lu-neg/Lu-pos and Lu-pos/Lu-neg ratios are presented in upper and lower panels, respectively. Data are from two independent cell preparations. (B) Icam-1, Met, and Hgf expression in Lu-neg, Lu-pos, and basal/myoepithelial cells, as determined by q-PCR. Cells were isolated from mammary glands of 8-day-pregnant mice. The q-PCR values were normalized to Gapdh expression and represent mean from two independent preparations. (C) Immunodetection of CD44v6 and K8 in cytocentrifuged Lu-neg and Lu-pos cells freshly isolated from mammary glands of mature virgin mice. In upper panels, nuclei were stained with DAPI. Bar, 15 μm.

From: Di-Cicco A, Petit V, Chiche A, Bresson L, Romagnoli M, Orian-Rousseau V, Vivanco Md, Medina D, Faraldo MM, Glukhova MA, Deugnier MA. Paracrine Met signaling triggers epithelial-mesenchymal transition in mammary luminal progenitors, affecting their fate.
Elife. 2015 Jul 13;4.

  • CD44v6 Antibody | 9A4 thumbnail image 1
  • Rat anti Mouse CD44v6
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1967C, E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Mouse CD44v6, clone 9A4 recognizes an epitope encoded by exon v6 on the variant portion of murine CD44. CD44v6 has been identified along with ICAM-1 as a co-receptor for Met and in mammary epithelia appears to act at the primary Met co-receptor (Di-Cicco et al2015).

      Rat anti Mouse CD44v6, clone 9A4 has been used successfully for the identification of CD44v6 expressing cells by immunofluorescence (Di-Cicco et al. 2015), western blotting (Lo et al.2012). immunohistochemistry (Weilenga et al. 1999) along with ELISA and flow cytometry (Weiss et al. 1997).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      GST-CD44v6 fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized female DA rats were fused with cells from the SP/0 myeloma.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD44v6 Antibody Formats

    Formats Clone Applications Sizes available
    CD44v6 Antibody : Purified 9A4 C, E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
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      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls


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