CD44v6 antibody | 9A4
Rat anti Mouse CD44v6
- Product Type
- Monoclonal Antibody
- Clone
- 9A4
- Isotype
- IgG1
- Specificity
- CD44v6
Rat anti Mouse CD44v6, clone 9A4 recognizes an epitope encoded by exon v6 on the variant portion of murine CD44. CD44v6 has been identified along with ICAM-1 as a co-receptor for Met and in mammary epithelia appears to act at the primary Met co-receptor (Di-Cicco et al. 2015). Rat anti Mouse CD44v6, clone 9A4 has been used successfully for the identification of CD44v6 expressing cells by immunofluorescence (Di-Cicco et al. 2015), western blotting (Lo et al. 2012). Immunohistochemistry (Weilenga et al. 1999) along with ELISA and flow cytometry (Weiss et al. 1997). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- GST-CD44v6 fusion protein.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from immunized female DA rats were fused with cells from the SP/0 myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Antigen Retrieval Buffer, pH8.0 |
References for CD44v6 antibody
-
Weiss JM et al. (1997) An essential role for CD44 variant isoforms in epidermal Langerhans cell and blood dendritic cell function.
J Cell Biol. 137 (5): 1137-47. -
Wielenga VJ et al. (1999) Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway.
Am J Pathol. 154 (2): 515-23. -
Katagiri, Y.U. et al. (1999) CD44 variants but not CD44s cooperate with beta1-containing integrins to permit cells to bind to osteopontin independently of arginine-glycine-aspartic acid, thereby stimulating cell motility and chemotaxis.
Cancer Res. 59: 219-26. -
Hebbard, L. et al. (2000) CD44 expression and regulation during mammary gland development and function.
J Cell Sci. 113: 2619-30. -
Rochman, M. et al. (2000) The CD44 receptor of lymphoma cells: structure-function relationships and mechanism of activation.
Cell Adhes Commun. 7: 331-47. -
Khaldoyanidi, S. et al. (2002) CD44 variant-specific antibodies trigger hemopoiesis by selective release of cytokines from bone marrow macrophages.
Blood. 99: 3955-61. -
Termeer, C. et al. (2003) Targeting dendritic cells with CD44 monoclonal antibodies selectively inhibits the proliferation of naive CD4+ T-helper cells by induction of FAS-independent T-cell apoptosis.
Immunology. 109: 32-40. -
Matrosova, V.Y. et al. (2004) Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.
Stem Cells. 22: 544-55.
View The Latest Product References
-
Serobyan, N. et al. (2005) Nicotinic acetylcholine receptor-mediated stimulation of endothelial cells results in the arrest of haematopoietic progenitor cells on endothelium.
Br J Haematol. 129: 257-65. -
Boon, E.M. et al. (2006) Activation of Wnt signaling in the intestinal mucosa of Apc +/min mice does not cause overexpression of the receptor tyrosine kinase Met.
Cancer Sci. 97: 710-5. -
Wallach-Dayan, S.B. et al. (2008) DNA vaccination with CD44 variant isoform reduces mammary tumor local growth and lung metastasis.
Mol Cancer Ther. 7: 1615-23. -
Lo, B. et al. (2012) Lkb1 regulates organogenesis and early oncogenesis along AMPK-dependent and -independent pathways.
J Cell Biol. 199: 1117-30. -
Nakagawa, H. et al. (2014) Loss of liver E-cadherin induces sclerosing cholangitis and promotes carcinogenesis.
Proc Natl Acad Sci U S A. 111(3):1090-5. -
Di-Cicco, A. et al. (2015) Paracrine Met signaling triggers epithelial-mesenchymal transition in mammary luminal progenitors, affecting their fate.
Elife. 2015 Jul 13;4. -
Shibata, W. et al. (2017) Helicobacter-induced gastric inflammation alters the properties of gastric tissue stem/progenitor cells.
BMC Gastroenterol. 17 (1): 145.
MCA1967
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