CD254 Antibody | IK22/5

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  • Rat anti Mouse CD254
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    IK22/5
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4711C, F, IF, IP, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA4711
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse CD254 antibody, clone IK22/5 recognises CD254, otherwise known as Receptor activator of nuclear factor kappa B ligand (RANKL) or TNF-related activation-induced cytokine (TRANCE), a member of the tumour necrosis factor ligand superfamily. CD254 is a cytokine that binds osteoprotegerin or RANK and plays an important role in bone metabolism, where it acts as an osteoclast differentiation and activation factor. Overproduction of CD254 is implicated in a range of degenerative bone diseases.

      CD254 is also expressed by T helper cells and augments the ability of dendritic cells to stimulate naïve T cell proliferation. It may play a role in regulating interactions between T cells and dendritic cells and in the regulation of T cell-dependent immune responses.

      Rat anti Mouse CD254 antibody, clone IK22/5 blocks ligand binding.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD254 Antibody Formats

    Formats Clone Applications Sizes available
    CD254 Antibody : Purified IK22/5 C, F, IF, IP, WB 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
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      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
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      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
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      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
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      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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