CD253 Antibody | N2B2

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  • Rat anti Mouse CD253
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    N2B2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4710GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA4710GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD253 antibody, clone N2B2 recognizes mouse CD253, also known as TNF-related apoptosis-inducing ligand or TRAIL, a member of the tumour necrosis factor ligand superfamily. When bound to the death receptors DR4 and DR5 caspase-8-dependent apoptosis is induced. However, CD253 might also be bound by the decoy receptor DcR1 which cannot induce apoptosis, and DcR2 which activates NF-kappa-B.

      Rat anti Mouse CD253 antibody, clone N2B2 has been reported to block NK cell cytotoxicity in vitro (Kayagaki et al. 1999).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Mouse CD253-transfected 2PK-3 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      apoptosis
      integral to membrane
      immune response
      tumor necrosis factor receptor binding
      extracellular space
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD253 Antibody Formats

    Formats Clone Applications Sizes available
    CD253 Antibody : Purified N2B2 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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