CD16 Antibody | AT154-2

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  • Rat anti Mouse CD16
  • Rat anti Mouse CD16:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AT154-2
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5998FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5998F
    MCA5998C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA5998
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.

      In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      HEK293F cell expressing CD16 and gamma chain
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
    • GO Terms
      antibody-dependent cellular cytotoxicity
      serotonin secretion
      neutrophil chemotaxis
      IgG receptor activity
      antigen processing and presentation of exogenous peptide antigen via MHC class I
      positive regulation of type IIa hypersensitivity
      phagocytosis, engulfment
      mast cell activation
      cell surface receptor linked signaling pathway
      positive regulation of phagocytosis
      positive regulation of type III hypersensitivity
      plasma membrane
      phagocytosis, recognition
      positive regulation of type I hypersensitivity
      positive regulation of tumor necrosis factor biosynthetic process
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Immunohistology - Frozen1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD16 Antibody Formats

    Formats Clone Applications Sizes available
    CD16 Antibody : Purified AT154-2 C, F 0.25 mg
    CD16 Antibody : FITC AT154-2 F 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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